Infection of Arabidopsis thaliana by Phytophthora parasitica and identification of variation in host specificity.
Infection of Arabidopsis thaliana by Phytophthora parasitica and identification of variation in host specificity.
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DOI：
10.1111/j.1364-3703.2010.00659.x
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发表时间：
2011-02
影响因子：
4.9
通讯作者：
Yan Wang;Yuling Meng;Meng Zhang;Xinmeng Tong;Qinhu Wang;Yi-Biao Sun;J. Quan;F. Govers;
中科院分区：
文献类型：
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作者：
Yan Wang;Yuling Meng;Meng Zhang;Xinmeng Tong;Qinhu Wang;Yi-Biao Sun;J. Quan;F. Govers;
卵菌病原体对多种重要的农业作物和自然生态系统造成严重破坏。它们代表了一组独特的植物病原体，在进化上与真正的真菌相去甚远。在这项研究中，我们建立了一个新的植物-卵菌病理系统，在该系统中，寄主范围广的病原菌寄生疫霉菌能够与模式植物拟南芥相互作用。接种寄生蜂游动孢子后3d内叶片出现浸水病斑，5d内形成大量孢子囊。细胞学鉴定表明，寄生疫霉发生附着性肿胀，并直接穿透表皮细胞，最好是在寄主细胞壁间的交界处。接种游动孢子后1d，表皮细胞和叶肉细胞均形成多个吸器样结构。对25个拟南星天牛生态型与6个寄生疫霉菌株的致病力测定结果表明，海桑寄主专化性与寄主专化性存在自然变异。大多数生态型对寄生疫霉Pp014、Pp016和Pp025高度敏感，但对Pp008和Pp009抗病，细胞壁沉积和主动防御反应细胞坏死频繁出现。基因表达和比较转录分析进一步证实了在拟南芥中表达上调的基因，这些基因是生物胁迫的特征。已建立的刺参A.thaliana-P.寄生病原菌系统扩展了研究卵菌-植物相互作用的模型系统，有助于全面了解疫霉的生物学和病理学。
Oomycete pathogens cause severe damage to a wide range of agriculturally important crops and natural ecosystems. They represent a unique group of plant pathogens that are evolutionarily distant from true fungi. In this study, we established a new plant-oomycete pathosystem in which the broad host range pathogen Phytophthora parasitica was demonstrated to be capable of interacting compatibly with the model plant Arabidopsis thaliana. Water-soaked lesions developed on leaves within 3 days and numerous sporangia formed within 5 days post-inoculation of P. parasitica zoospores. Cytological characterization showed that P. parasitica developed appressoria-like swellings and penetrated epidermal cells directly and preferably at the junction between anticlinal host cell walls. Multiple haustoria-like structures formed in both epidermal cells and mesophyll cells 1 day post-inoculation of zoospores. Pathogenicity assays of 25 A. thaliana ecotypes with six P. parasitica strains indicated the presence of a natural variation in host specificity between A. thaliana and P. parasitica. Most ecotypes were highly susceptible to P. parasitica strains Pp014, Pp016 and Pp025, but resistant to strains Pp008 and Pp009, with the frequent appearance of cell wall deposition and active defence response-based cell necrosis. Gene expression and comparative transcriptomic analysis further confirmed the compatible interaction by the identification of up-regulated genes in A. thaliana which were characteristic of biotic stress. The established A. thaliana-P. parasitica pathosystem expands the model systems investigating oomycete-plant interactions, and will facilitate a full understanding of Phytophthora biology and pathology.