The Bacterial ZapA-like Protein ZED Is Required for Mitochondrial Division
The Bacterial ZapA-like Protein ZED Is Required for Mitochondrial Division
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DOI：
10.1016/j.cub.2009.07.035
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发表时间：
2009-09-15
期刊：
影响因子：
9.2
通讯作者：
Kuroiwa, Tsuneyoshi
中科院分区：
文献类型：
--
作者：
Yoshida, Yamato;Kuroiwa, Haruko;Kuroiwa, Tsuneyoshi
包括FtsZ在内的细菌细胞分裂系统存在于整个原核生物中。线粒体起源于一种内共生的α -变形菌祖先，并通过分裂进行增殖[2-4]。然而，线粒体分裂系统是如何从细菌分裂中建立起来的尚不清楚。在这里，我们从原始红藻Cyanidioschyzon merolae中分离出完整的线粒体分裂（MD）机制，并鉴定出一种细菌zapa样蛋白ZED，它可以用FtsZ收缩MD机制的基础结构。ZED包含一个预测的线粒体转运信号和两个线圈区，与细菌分裂蛋白ZapA[5]部分同源。细胞学研究表明，ZED积累形成一个环形结构，与FtsZ在内膜下共定位。ZED蛋白在线粒体分裂前表达。短型ZED （S-ZED）随后出现在线粒体收缩期。蛋白-蛋白相互作用分析和对ZED的反义瞬时表达表明，S-ZED与FtsZ1相互作用构成了线粒体分裂机制的基础结构。我们还证明了细菌ZapA和线粒体ZED之间令人信服的功能相似性，这表明细菌细胞分裂系统在进化过程中通过细菌分裂蛋白的重塑被纳入了MD机制。
Bacterial cell division systems that include FtsZ are found throughout prokaryotes [1]. Mitochondria arose from an endosymbiotic alpha-proteobacterial ancestor and proliferate by division [2-4]. However, how the mitochondrial division system was established from bacterial division is not clear. Here, we have isolated intact mitochondrial division (MD) machineries from the primitive red alga Cyanidioschyzon merolae and identified a bacterial ZapA-like protein, ZED, that constricts the basal structure of MD machinery with FtsZ. ZED contains a predicted mitochondrial transit signal and two coiled-coil regions and has partial homology with the bacterial division protein ZapA [5]. Cytological studies revealed that ZED accumulates to form a ring structure that colocalizes with FtsZ beneath the inner membrane. ZED proteins are expressed just before mitochondrial division. The short-form ZED (S-ZED) then appears at the mitochondrial constriction phase. Protein-protein interaction analysis and transient expression of antisense against ZED showed that S-ZED interacts with FtsZ1 to constitute the basal structure of the MD machinery and is required for mitochondrial division. We also demonstrate compelling functional similarity between bacterial ZapA and mitochondrial ZED, suggesting that the bacterial cell division system was incorporated into the MD machinery with remodeling of bacterial division proteins during evolution.