Contrasting modes of diversification in the Aux/IAA and ARF gene families
Contrasting modes of diversification in the Aux/IAA and ARF gene families
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DOI：
10.1104/pp.104.039669
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发表时间：
2004-07-01
期刊：
影响因子：
7.4
通讯作者：
Reed, JW
中科院分区：
文献类型：
--
作者：
Remington, DL;Vision, TJ;Reed, JW
拟南芥全基因组序列的获得为联合收割机结合系统发育和基因组学方法研究植物基因家族的进化提供了机会。Aux/IAA和ARF基因家族在拟南芥中分别由29个和23个位点组成，编码相互作用以介导生长素反应并调节植物形态发育的各个方面的蛋白质。我们开发的方案，基因组增殖的Aux/IAA和ARF家庭相结合的系统发育分析与信息之间的关系，每个位点和先前确定的重复基因组片段在拟南芥。这一分析表明，这两个基因家族至少可以追溯到陆地植物的起源，并且主要的Aux/IAA和ARF谱系起源于单子叶-真双子叶分化之前。我们发现，现存的Aux/IAA基因座主要是通过节段性重复事件，形成鲜明对比的ARF家族和基因家族在拟南芥中的一般模式增殖。Aux/IAA复制的不寻常模式的可能解释包括蛋白质和途径之间复杂的相互作用所施加的进化限制，或长距离顺式调控序列的存在。这两个基因家族的古老性和Aux/IAA多样化的不寻常模式对于理解这些基因的功能和进化作用具有许多潜在的意义。
The complete genomic sequence for Arabidopsis provides the opportunity to combine phylogenetic and genomic approaches to study the evolution of gene families in plants. The Aux/IAA and ARF gene families, consisting of 29 and 23 loci in Arabidopsis, respectively, encode proteins that interact to mediate auxin responses and regulate various aspects of plant morphological development. We developed scenarios for the genomic proliferation of the Aux/IAA and ARF families by combining phylogenetic analysis with information on the relationship between each locus and the previously identified duplicated genomic segments in Arabidopsis. This analysis shows that both gene families date back at least to the origin of land plants and that the major Aux/IAA and ARF lineages originated before the monocot-eudicot divergence. We found that the extant Aux/IAA loci arose primarily through segmental duplication events, in sharp contrast to the ARF family and to the general pattern of gene family proliferation in Arabidopsis. Possible explanations for the unusual mode of Aux/IAA duplication include evolutionary constraints imposed by complex interactions among proteins and pathways, or the presence of long-distance cis-regulatory sequences. The antiquity of the two gene families and the unusual mode of Aux/IAA diversification have a number of potential implications for understanding both the functional and evolutionary roles of these genes.