Molecular cloning and characterization of a cDNA encoding ent-cassa-12,15-diene synthase, a putative diterpenoid phytoalexin biosynthetic enzyme, from suspension-cultured rice cells treated with a chitin elicitor
Molecular cloning and characterization of a cDNA encoding ent-cassa-12,15-diene synthase, a putative diterpenoid phytoalexin biosynthetic enzyme, from suspension-cultured rice cells treated with a chitin elicitor
复制标题
DOI：
10.1046/j.1365-313x.2003.01926.x
复制
发表时间：
2004-01-01
期刊：
影响因子：
7.2
通讯作者：
Yamane, H
中科院分区：
文献类型：
--
作者：
Cho, EM;Okada, A;Yamane, H
我们从甲壳素激发子处理的悬浮培养水稻细胞中分离并鉴定了编码一种新的二萜环化酶OsDTC1的基因。OsDTC1是一种Et-Cassa-12，15-二烯合成酶，被认为在水稻二萜类植物保卫素的生物合成中起着关键作用。在紫外线(UV)辐照的水稻叶片中也证实了OsDTC1mRNA的表达。此外，我们还在甲壳素诱导的悬浮培养水稻细胞和UV辐照的水稻叶片中鉴定了(-)-植卡烷的二萜烃前体ent-Cassa-12，15-diene是一种内源化合物。本研究分离到的OsDTC1c DNA将成为研究水稻(-)-植卡烷生物合成调控机制的有用工具。
We have isolated and characterized a cDNA encoding a novel diterpene cyclase, OsDTC1, from suspension-cultured rice cells treated with a chitin elicitor. OsDTC1 functions as ent-cassa-12,15-diene synthase, which is considered to play a key role in the biosynthesis of (-)-phytocassanes recently isolated as rice diterpenoid phytoalexins. The expression of OsDTC1 mRNA was also confirmed in ultraviolet (UV)-irradiated rice leaves. In addition, we identified ent-cassa-12,15-diene, a putative diterpene hydrocarbon precursor of (-)-phytocassanes, as an endogenous compound in the chitin-elicited suspension-cultured rice cells and the UV-irradiated rice leaves. The OsDTC1 cDNA isolated here will be a useful tool to investigate the regulatory mechanisms of the biosyntheis of (-)-phytocassanes in rice.