Reticulated platelets as a marker of platelet recovery after allogeneic stem cell transplantation
Reticulated platelets as a marker of platelet recovery after allogeneic stem cell transplantation
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DOI：
10.1111/j.1751-553x.2007.00993.x
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发表时间：
2008-12-01
影响因子：
3
通讯作者：
Marianska, B.
中科院分区：
文献类型：
--
作者：
Michur, H.;Maslanka, K.;Marianska, B.
网织血小板（RP）是血液中最年轻的血小板形式，反映了骨髓血小板产生的速率。在本研究中，我们使用流式细胞术分析来确定接受异基因干细胞移植的患者中RP的百分比。我们研究了10例HLA相合同胞移植后的患者：5例急性髓性白血病（AML），4例慢性髓性白血病（CML），1例骨髓增生异常综合征（MDS）。在接受检查的患者中，4例患者接受了异基因骨髓移植，6例患者接受了外周血干细胞移植。观察到在随后的10-26天内，所有患者（13.6 +/- 6.4%）的RP初始降低百分比（2.9 +/- 1.7%;平均值+/- SD）显著更高（P = 0.0109）。RP百分比峰值出现在外周血小板计数恢复至平均45.6 × 10（9）/l之前3天。我们发现AML和CML患者之间的RP%没有差异，但我们确实观察到CML患者的RP百分比平均比AML患者早7天增加。RP百分比的升高反映了骨髓再生的增加，并且可以被认为是接受异基因干细胞移植的患者中血小板生成恢复的额外标志物。
Reticulated platelets (RP) are the youngest forms of platelets in blood and reflect the rate of bone marrow platelet production. In the present study, we used flow cytometric analysis to determine the percentage of RPs in patients undergoing allogeneic stem cell transplantation. We investigated 10 patients after transplantation from HLA identical siblings: five with acute myeloid leukemia (AML), four with chronic myeloid leukemia (CML), and one patient with myelodysplastic syndrome (MDS). Of the patients examined, four patients underwent allogeneic bone marrow transplantation and six patients underwent peripheral blood stem cell transplantation. It was observed that the initially reduced percentage of RPs (2.9 +/- 1.7%; mean +/- SD) was significantly higher (P = 0.0109) in all patients (13.6 +/- 6.4%) in the following 10-26 days. The RP percentage peak preceded the recovery of peripheral platelet count up to 45.6 x 10(9)/l on average by 3 days. We found no difference in RP% between the AML and CML patients but we did observe that in CML patients the RP percentage increased on average 7 days earlier than in AML patients. The elevated RP percentage reflects increased bone marrow regeneration and can be considered an additional marker of thrombopoietic recovery in the patients undergoing allogeneic stem cell transplantation.