Avidity and subtyping of specific antibodies applied to the indirect assessment of heterologous protection against Foot-and-Mouth Disease Virus in cattle
Avidity and subtyping of specific antibodies applied to the indirect assessment of heterologous protection against Foot-and-Mouth Disease Virus in cattle
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DOI：
10.1016/j.vaccine.2012.09.011
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发表时间：
2012-11-06
期刊：
影响因子：
5.5
通讯作者：
Victoria Capozzo, Alejandra
中科院分区：
文献类型：
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作者：
Angeles Lavoria, Maria;Di-Giacomo, Sebastian;Victoria Capozzo, Alejandra
口蹄疫病毒（FMDV）株间异源应答的血清学评价主要采用病毒中和试验（VNT）、液相阻断ELISA和补体固定试验。本研究建立了两种高通量ELISA技术，avidity和IgG亚型ELISA，用于进一步表征牛在接种和攻毒期间的异源抗体反应。这两种检测方法均应用于一组先前鉴定的动物血清，这些血清接种了A24 Cruzeiro/Brazil/55 （A24 Cruzeiro）单价FMDV灭活疫苗，并用异源a/ Argentina/2001 （a/ Arg/01）菌株攻毒。单稀释亲和度ELISA检测结果显示，A/Arg/01攻毒保护组对该异种菌株的亲和度高于未保护组。抗a /Arg/01血清中和滴度低甚至检测不到的动物通过异源攻击后，其IgG1/IgG2比值高于未受保护的动物。在这项研究中，三种评估（VNT和两种elisa）区分了受保护和未受保护的动物对异源攻击的反应。这些技术的结合可用于补充目前的间接血清学疫苗匹配评估。这些定性参数的测量可能为了解牛口蹄疫异源反应和诱导交叉保护的机制提供额外的信息。(C) 2012 Elsevier Ltd.版权所有。
Serological assessment of the heterologous response among Foot-and-Mouth Disease Virus (FMDV) strains is mainly performed by virus neutralization test (VNT), liquid phase blocking ELISA and complement fixation assay. In this study two high-throughput ELISA techniques, avidity and IgG subtype ELISA, were developed and used to further characterize heterologous antibody responses in cattle during vaccination and challenge. Both assays were applied to a set of previously characterized sera from animals immunized with an inactivated A24 Cruzeiro/Brazil/55 (A24 Cruzeiro) strain monovalent FMDV vaccine and challenged with the heterologous A/Argentina/2001 (A/Arg/01) strain. Single dilution avidity ELISA assessment showed that animals that were protected against A/Arg/01 challenge had higher avidity antibodies to this heterologous strain than non-protected cattle. Animals with low or even undetectable anti-A/Arg/01 serum-neutralizing titers that passed the heterologous challenge presented higher IgG1/IgG2 ratio than non-protected animals. In this study, the three assessments (VNT and both ELISAs) discriminated between protected and not protected animals against a heterologous challenge. The combination of these techniques may be applied to complement current indirect serological vaccine-matching assessments. The measurement of these qualitative parameters may provide additional information to understand the mechanisms underlying FMD heterologous responses and the induction of cross-protection in cattle. (C) 2012 Elsevier Ltd. All rights reserved.