Translational diffusion and fluid domain connectivity in a two-component, two-phase phospholipid bilayer.
Translational diffusion and fluid domain connectivity in a two-component, two-phase phospholipid bilayer.
复制标题
双组分、两相磷脂双层中的平移扩散和流体域连接。
DOI：
10.1016/s0006-3495(89)82733-x
复制
发表时间：
1989
影响因子：
3.4
通讯作者：
Thompson,TE
中科院分区：
文献类型：
--
作者：
Vaz,WL;Melo,EC;Thompson,TE
二维连通性检查的混合双分子层的二肉豆蔻酰磷脂酰胆碱（DMPC）和二硬脂酰磷脂酰胆碱（DSPC）作为一个功能的组合物和温度在恒压下使用荧光恢复后的光漂白（FRAP）方法。这些磷脂混合物表现出具有凝胶相和液晶相共存的大区域的周向行为。二月桂酰磷脂酰乙醇胺通过其头基中的氨基功能共价连接到荧光硝基苯并二唑基（NBD-DLPE）在这项研究中被用作荧光探针，因为它被发现分配几乎完全在液晶相中。这些研究的结果表明，在相当宽的浓度范围内，连接线接近相图上的液相线。在该范围内，包含约20%系统的凝胶相与包含80%系统的液晶相断开。这一结果的影响进行了讨论域的形状和组织的生物膜组件。
The two-dimensional connectivity is examined for mixed bilayers of dimyristoyl phosphatidylcholine (DMPC) and distearoyl phosphatidylcholine (DSPC) as a function of composition and temperature at constant pressure using the fluorescence recovery after photobleaching (FRAP) method. These phospholipid mixtures exhibit peritectic behavior with a large region in which both gel and liquid crystalline phases coexist. Dilauroyl phosphatidylethanolamine covalently linked through the amino function in its head group to the fluorescent nitrobenzodiazolyl group (NBD-DLPE) was used as the fluorescent probe in this study, because it was found to partition almost exclusively in the liquid crystalline phase. The results of these studies show the line of connectivity to be close to the liquidus line on the phase diagram over a rather broad range of concentrations. In this range, a gel phase comprising approximately 20% of the system disconnects a liquid crystalline phase comprising 80% of the system. The implications of this result are discussed for domain shape and the organization of biological membrane components.