Development of rapid and simultaneous quantitative method for green tea catechins on the bioanalytical study using UPLC/ESI-MS
Development of rapid and simultaneous quantitative method for green tea catechins on the bioanalytical study using UPLC/ESI-MS
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DOI：
10.1002/bmc.2740
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发表时间：
2013-01-01
影响因子：
1.8
通讯作者：
Yamada, Shizuo
中科院分区：
文献类型：
--
作者：
Misaka, Shingen;Kawabe, Keisuke;Yamada, Shizuo
建立了超高效液相色谱-电喷雾质谱法同时测定绿色茶中儿茶素的快速定量分析方法。总分析运行时间为3.5？以内标法测定大鼠血浆中的（-）-表儿茶素（EC）、（-）-表儿茶素-3-O-没食子酸酯（ECG）、（-）-表没食子儿茶素（EGC）、（-）-表没食子儿茶素-3-O-没食子酸酯（EGCG）和杨梅素。在105000？ng/mL。该方法的日间和日间精密度（相对标准偏差）和准确度（百分比偏差）均低于10%。血浆中EC、EGC、ECG和EGCG的平均提取回收率为68.5%~ 86.5%，定量下限为10？ng/mL，信噪比>10。该方法已成功应用于大鼠静脉和灌胃给予绿色茶提取物后儿茶素类的药代动力学研究。血浆浓度的四个儿茶素检测到524？h，儿茶素类药物的药代动力学参数与前人研究结果一致。从这些发现，结合高生产率和精度，开发的方法可以是一个可靠的和可重复的工具，儿茶素的药代动力学特性的评价。版权所有（c）2012约翰威利父子有限公司
A rapid and quantitative analytical method for the simultaneous determination of green tea catechins using ultra-performance liquid chromatography/electrospray ionizationmass spectrometry was developed. Total analytical run time was 3.5?min for the detection of (-)-epicatechin (EC), (-)-epicatechin-3-O-gallate (ECG), (-)-epigallocatechin (EGC), (-)-epigallocatechin-3-O-gallate (EGCG) and myricetin as the internal standard (IS) in rat plasma. The calibration curves were linear over the range of 105000?ng/mL for all the catechins. The inter- and intra-day precision (relative standard deviation) and accuracy (percentage deviation) of the method were both lower than 10%. The average extraction recoveries in plasma ranged from 68.5 to 86.5%, and the lower limits of quantification of EC, EGC, ECG and EGCG were 10?ng/mL with a signal-to-noise ratio of >10. The assay developed was successfully applied to a pharmacokinetic study of catechins following intravenous and intragastric administrations of green tea extract in rats. Plasma concentrations of four catechins were detected up to 524?h after administration, and the pharmacokinetic parameters of catechins were in agreement with previous studies. From these findings, taken together with the high productivity and precision, the developed method could be a reliable and reproducible tool for the evaluation of pharmacokinetic properties of catechins. Copyright (c) 2012 John Wiley & Sons, Ltd.