The Thellungiella salsuginea tonoplast aquaporin TsTIP1;2 functions in protection against multiple abiotic stresses.
The Thellungiella salsuginea tonoplast aquaporin TsTIP1;2 functions in protection against multiple abiotic stresses.
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DOI：
10.1093/pcp/pct166
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发表时间：
2014
影响因子：
4.9
通讯作者：
Lili Wang;Anping Chen;Nai-Qin Zhong;Ning Liu;Xiao-Min Wu;F. Wang;Chun-Lin Yang;M. Romero;
中科院分区：
文献类型：
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作者：
Lili Wang;Anping Chen;Nai-Qin Zhong;Ning Liu;Xiao-Min Wu;F. Wang;Chun-Lin Yang;M. Romero;
研究极端微生物植物的水通道蛋白（AQP）膜通道可以增加我们对植物对高盐、干旱或其他条件的耐受性的理解。本研究从盐生植物盐芥中克隆了一个液泡膜AQP基因（TsTIP 1;2），并对其生物学功能进行了研究。TsTIP 1;2转录本在几个器官中积累到高水平，在多种外部刺激下增加。拟南芥中TsTIP 1;2的异位过表达显著提高了植物对干旱、盐和氧化胁迫的耐受性。TsTIP 1;2在非洲爪蟾卵母细胞中表达后具有水通道活性。TsTIP 1;2还能够在氧化应激反应中将H-2 O-2分子导入酵母细胞。TsTIP1; 2对Na（+）不具渗透性，但在高盐条件下，它能促进Na（+）通过间接途径进入植物细胞液泡。总的来说，这些数据表明TsTIP 1;2可以介导H-2 O和H-2 O-2的跨膜传导，并且可能作为T.在高度紧张的栖息地中的盐芥。
Examination of aquaporin (AQP) membrane channels in extremophile plants may increase our understanding of plant tolerance to high salt, drought or other conditions. Here, we cloned a tonoplast AQP gene (TsTIP1;2) from the halophyte Thellungiella salsuginea and characterized its biological functions. TsTIP1;2 transcripts accumulate to high levels in several organs, increasing in response to multiple external stimuli. Ectopic overexpression of TsTIP1;2 in Arabidopsis significantly increased plant tolerance to drought, salt and oxidative stresses. TsTIP1;2 had water channel activity when expressed in Xenopus oocytes. TsTIP1;2 was also able to conduct H₂O₂ molecules into yeast cells in response to oxidative stress. TsTIP1;2 was not permeable to Na(+) in Xenopus oocytes, but it could facilitate the entry of Na(+) ions into plant cell vacuoles by an indirect process under high-salinity conditions. Collectively, these data showed that TsTIP1;2 could mediate the conduction of both H₂O and H₂O₂ across membranes, and may act as a multifunctional contributor to survival of T. salsuginea in highly stressful habitats.