A population genetic framework for the study of invasive diseases caused by serotype b strains of Haemophilus influenzae.
A population genetic framework for the study of invasive diseases caused by serotype b strains of Haemophilus influenzae.
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用于研究 b 型流感嗜血杆菌菌株引起的侵袭性疾病的群体遗传框架。
DOI：
10.1073/pnas.82.15.5078
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发表时间：
1985
影响因子：
11.1
通讯作者：
Selander,RK
中科院分区：
文献类型：
--
作者：
Musser,JM;Granoff,DM;Pattison,PE;Selander,RK
通过16种代谢酶的电泳迁移率、外膜蛋白（OMP）的NaDodSO4/PAGE模式和生物型，研究了177株主要从美国侵袭性疾病儿童中恢复的血清b型流感嗜血杆菌。根据酶位点的等位基因谱，鉴定出32种不同的多位点基因型（电泳型，ETs）。共鉴定出28种OMP型和5种生物型，但仅有55种ET、OMP型和生物型的独特组合。在广泛分离的地理区域和超过40年的时间内，特征的强非随机关联和具有相同特性的分离株的恢复表明流感嗜血杆菌的种群结构基本上是克隆的。对非血清b型分离株的检测表明，血清b型克隆是整个物种中基因型的一个有限子集。目前，美国的大多数侵袭性流感嗜血杆菌疾病是由两种相关et的血清型b株引起的，更具体地说，其中大部分可归因于两个以OMP型为标志的亚克隆。有证据表明，自1939-1954年以来，ET-1/OMP 1H/ I型生物亚克隆的频率在美国急剧增加。流感嗜血杆菌种群在克隆组成上存在显著的时间差异，这一假设得到了美国和荷兰种群遗传结构存在重大差异的证据的支持。
One hundred seventy-seven isolates of serotype b Haemophilus influenzae recovered largely from children with invasive disease in the United States were characterized by the electrophoretic mobilities of 16 metabolic enzymes, the NaDodSO4/PAGE pattern of outer-membrane proteins (OMP), and biotype. Thirty-two distinctive multilocus genotypes (electrophoretic types, ETs) were distinguished on the basis of allele profiles at the enzyme loci. Twenty-eight OMP types and five biotypes were identified, but only 55 distinctive combinations of ET, OMP type, and biotype were represented. The strong nonrandom associations of characters and the recovery of isolates with identical properties in widely separated geographic regions and over a 40-year period suggest that the population structure of H. influenzae is basically clonal. Examination of nonserotype b isolates indicated that clones of serotype b are a restricted subset of the genotypes in the species as a whole. Currently, most of the invasive H. influenzae disease in the United States is caused by serotype b strains of two related ETs, and, more specifically, much of it is attributable to two subclones marked by OMP type. There is evidence that the frequency of the ET-1/OMP 1H/biotype I subclone has increased dramatically in the United States since the 1939-1954 period. The hypothesis that populations of H. influenzae are subject to marked temporal variation in clonal composition is supported by evidence of major differences in the genetic structure of populations in the United States and the Netherlands.