Dielectrophoresis and dielectrophoretic impedance detection of adenovirus and rotavirus
Dielectrophoresis and dielectrophoretic impedance detection of adenovirus and rotavirus
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DOI：
10.7567/jjap.55.017001
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发表时间：
2016-01-01
影响因子：
1.5
通讯作者：
Suehiro, Junya
中科院分区：
文献类型：
--
作者：
Nakano, Michihiko;Ding, Zhenhao;Suehiro, Junya
本研究的目的是使用介电泳阻抗测量（DEPIM）对致病病毒，即腺病毒和轮状病毒进行电检测。DEPIM包括两个同时进行的过程：介电泳捕获的目标和测量的阻抗变化和电导增加的数量被困的目标。这是首次将最初开发用于检测悬浮在水溶液中的细菌的DEPIM应用于病毒检测。病毒的介电特性也被研究在其介电电泳行为方面。虽然它们的介电性质与细菌的介电性质不同，但捕获的病毒以类似于细菌检测的方式增加了微电极的电导。我们证明了在60 s内的病毒的电检测浓度低至70 ng/ml的腺病毒和50 ng/ml的轮状病毒。(C)2016日本应用物理学会
The aim of this study is the electrical detection of pathogenic viruses, namely, adenovirus and rotavirus, using dielectrophoretic impedance measurement (DEPIM). DEPIM consists of two simultaneous processes: dielectrophoretic trapping of the target and measurement of the impedance change and increase in conductance with the number of trapped targets. This is the first study of applying DEPIM, which was originally developed to detect bacteria suspended in aqueous solutions, to virus detection. The dielectric properties of the viruses were also investigated in terms of their dielectrophoretic behavior. Although their estimated dielectric properties were different from those of bacteria, the trapped viruses increased the conductance of the microelectrode in a manner similar to that in bacteria detection. We demonstrated the electrical detection of viruses within 60 s at concentrations as low as 70 ng/ml for adenovirus and 50 ng/ml for rotavirus. (C) 2016 The Japan Society of Applied Physics