Ep3 and Ep4 receptor mRNA expression in peptidergic cell groups of the rat parabrachial nucleus
Ep3 and Ep4 receptor mRNA expression in peptidergic cell groups of the rat parabrachial nucleus
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DOI：
10.1016/j.neuroscience.2004.03.042
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发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Blomqvist, A
中科院分区：
文献类型：
--
作者：
Engblom, D;Ek, M;Blomqvist, A
本研究检测了前列腺素E-2受体EP3和EP4亚型在脑干臂旁神经元中的分布，其特点是神经肽的表达。用双标记原位杂交方法，我们发现在背侧亚核和中央外侧亚核中表达前强啡肽原的神经元表达EP3受体mRNA。这些受体在外侧亚核的前脑啡肽原、降钙素基因相关肽和速激肽原mRNA阳性神经元中也有表达，而在该亚核的前强啡肽原mRNA表达的神经元则为EP受体阴性。此外，EP3受体在Kolliker-Fuse核的一些脑啡肽能神经元中也有表达。位于上外侧亚核中央的CCK能神经元表达EP4受体mRNA，而位于较外围的神经元表达EP3受体。结合以往的研究结果表明，离散的多肽能细胞群将伤害性信息和/或内脏传入信息传递到不同的脑干和前脑区域，本结果提示臂旁核内这一信息的处理受到前列腺素E-2的影响。最近的研究表明，在外周免疫攻击后，前列腺素E-2被释放到大脑中；因此，臂旁核可能是外周炎症事件的体液信号与由相同外周过程引发的神经元信号相互作用的区域。(C)2004年IBRO。爱思唯尔有限公司出版。保留所有权利。
This study examines the distribution of prostaglandin E-2 receptors of subtype EP3 and EP4 among brain stem parabrachial neurons that were characterized with respect to their neuropeptide expression. By using a dual-labeling in situ hybridization method, we show that preprodynorphin mRNA expressing neurons in the dorsal and central lateral subnuclei express EP3 receptor mRNA. Such receptors are also expressed in preproenkephalin, calcitonin gene related peptide and preprotachykinin mRNA positive neurons in the external lateral subnucleus, whereas preprodynorphin mRNA expressing neurons in this subnucleus are EP receptor negative. In addition, EP3 receptor expression is seen among some enkephalinergic neurons in the Kolliker-Fuse nucleus. Neurons in the central part of the cholecystokininergic population in the regions of the superior lateral subnucleus express EP4 receptor mRNA, whereas those located more peripherally express EP3 receptors. Taken together with previous findings showing that discrete peptidergic cell groups mediate nociceptive and/or visceral afferent information to distinct brain stem and forebrain regions, the present results suggest that the processing of this information in the parabrachial nucleus is influenced by prostaglandin E-2. Recent work has shown that prostaglandin E-2 is released into the brain following peripheral immune challenge; hence, the parabrachial nucleus may be a region where humoral signaling of peripheral inflammatory events may interact with neuronal signaling elicited by the same peripheral processes. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.