In vivo effects of 17β-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart
In vivo effects of 17β-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart
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DOI：
10.1016/j.mce.2014.03.005
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发表时间：
2014-05-05
影响因子：
4.1
通讯作者：
Isenovic, Esma R.
中科院分区：
文献类型：
--
作者：
Obradovic, Milan;Stewart, Alan J.;Isenovic, Esma R.
本实验研究了雌二醇对大鼠心肌Na ~+/K ~+-ATP酶功能的调节作用。成年雄性Wistar大鼠用雌二醇（40 μ g/kg，i. p.）24小时后处死动物并切除心脏。给予雌二醇后，心脏Na+/K（+）ATP酶活性、α 1亚基表达和α 1亚基磷酸化显著增加。这些动物的地高辛样免疫反应因子水平也显著降低。Na+水平也显著降低，但仍在正常生理范围内，这突出了用雌二醇处理后Na+/K+-ATP酶平衡离子组成的能力。雌二醇治疗的大鼠还显示蛋白激酶B（Akt）和细胞外信号调节激酶1/2（ERK 1/2）的磷酸化增加。因此，我们认为Akt和/或ERK 1/2在雌二醇介导的大鼠心脏Na+/K+-ATP酶表达和活性的调节中起作用。(C)2014爱思唯尔爱尔兰有限公司版权所有。
In this study the in vivo effects of estradiol in regulating Na+/K+-ATPase function in rat heart was studied. Adult male Wistar rats were treated with estradiol (40 mu g/kg, i.p.) and after 24 h the animals were sacrificed and the heart excised. Following estradiol administration, cardiac Na+/K(+)ATPase activity, expression of the alpha l subunit, and phosphorylation of the al subunit were significantly increased. These animals also had significantly decreased levels of digoxin-like immunoreactive factor(s). Na+ levels were also significantly reduced but to a level that was still within the normal physiological range, highlighting the ability of the Na+/K+-ATPase to balance the ionic composition following treatment with estradiol. Estradiol treated rats also showed increased phosphorylation of protein kinase B (Akt), and extracellular-signal-regulated kinase 1/2 (ERK1/2). We therefore suggest a role for Akt and/or ERK1/2 in estradiol-mediated regulation of cardiac Na+/K+-ATPase expression and activity in rat heart. (C) 2014 Elsevier Ireland Ltd. All rights reserved.