EFFECTS OF GROWTH-FACTORS ON AN INTESTINAL EPITHELIAL-CELL LINE - TRANSFORMING GROWTH-FACTOR-BETA INHIBITS PROLIFERATION AND STIMULATES DIFFERENTIATION
EFFECTS OF GROWTH-FACTORS ON AN INTESTINAL EPITHELIAL-CELL LINE - TRANSFORMING GROWTH-FACTOR-BETA INHIBITS PROLIFERATION AND STIMULATES DIFFERENTIATION
复制标题
DOI：
10.1016/0006-291x(87)91481-1
复制
发表时间：
1987-02-13
影响因子：
3.1
通讯作者：
PODOLSKY, DK
中科院分区：
文献类型：
--
作者：
KUROKOWA, M;LYNCH, K;PODOLSKY, DK
表皮生长因子、转化生长因子的作用。（tgf . β .）和其他生长因子对大鼠肠隐窝上皮（IEC-6）细胞系增殖和分化的影响。胰岛素、胰岛素样生长因子（IGF）、血小板衍生生长因子（PDGF）、表皮生长因子（EGF）和2%胎牛血清（FCS）分别刺激[3H]胸苷的掺入量达到1.4 ~ 2.4倍。当FCS中添加胰岛素、IGF-I或PDGF而不添加EGF时，观察到添加性刺激。tgf . β强烈抑制IEC-6与[3H]-胸腺嘧啶的结合。当浓度为.simeq时，对掺入的抑制作用大于80%。2.0点。IEC-6细胞结合4.1 .+-。1.5的同学们。104分子tgf。/细胞，似乎只有一类高亲和力受体(Kd .simeq。0.5点)。TGF.beta。抑制作用不受胰岛素或igf - 1存在的影响，这表明它在这些生长因子刺激[3H]-胸腺嘧啶结合的步骤之后抑制增殖。TGF.beta。FCS诱导的刺激也减少了65%。相反，EGF降低tgf . β。抑制60%。经tgf - β处理18小时后，IEC-6细胞表现出蔗糖酶活性。这些发现表明tgf。可能抑制肠上皮细胞的增殖活性，促进分化功能的发展。
The effects of epidermal growth factor, transforming growth factor .beta. (TGF.beta.) and other growth factors on the proliferation and differentiation of a cell line derived from rat intestinal crypt epithelium (IEC-6) were defined. Incorporation of [3H]thymidine was stimulated 1.4-2.4 fold by insulin, insulin like growth factor (IGF), platelet derived growth factor (PDGF), epidermal growth factor (EGF) and 2% fetal calf serum (FCS) respectively. Additive stimulation was observed when FCS was supplemented by insulin, IGF-I or PDGF but not EGF. Incorporation of [3H]-thymidine by IEC-6 was strongly inhibited by TGF.beta. with greater than 80% inhibition of incorporation at concentration .simeq. 2.0 pM. IEC-6 cells bound 4.1 .+-. 1.5 .times. 104 molecules TGF.beta./cell and appeared to have only a single class of high affinity receptors (Kd .simeq. 0.5 pM). TGF.beta. inhibition was unaffected by the presence of insulin or IGF-I suggesting it inhibits proliferation at a step subsequent to that at which these growth factors stimulate [3H]-thymidine incorporation. TGF.beta. also reduced the stimulation induced by FCS by 65%. In contrast EGF reduced TGF.beta. inhibition by 60%. IEC-6 cells demonstrated the appearance of sucrase activity after > 18 hours treatment with TGF.beta.. These findings suggest that TGF.beta. may inhibit proliferative activity and promote the development of differentiated function in intestinal epithelial cells.