ACTIVATION OF A PLANT GENE BY T-DNA TAGGING - AUXIN-INDEPENDENT GROWTH-INVITRO (Retracted Article)
ACTIVATION OF A PLANT GENE BY T-DNA TAGGING - AUXIN-INDEPENDENT GROWTH-INVITRO (Retracted Article)
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DOI：
10.1126/science.1455228
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发表时间：
1992-11-20
期刊：
影响因子：
56.9
通讯作者：
WALDEN, R
中科院分区：
文献类型：
--
作者：
HAYASHI, H;CZAJA, I;WALDEN, R
将具有产生显性突变潜力的转移 DNA (T-DNA) 标记载体与共培养的根癌农杆菌和原生质体一起使用，以标记参与植物生长物质生长素作用的基因。根据转基因愈伤组织在培养基中缺乏生长素的情况下生长的能力来选择它们。在一项实验中，回收了 12 个表现出这种表型的愈伤组织，其中 11 个能够再生为植物。在一种经过详细研究的植物中，在缺乏生长素的情况下原生质体分裂与单个 T-DNA 插入片段在遗传上是共分离的。由从突变体中拯救出来的 6.4 KB 植物基因组 DNA 序列编码的信使 RNA 相对于未转化植物来说是过表达的。基因组 DNA 以及同源互补 DNA 一旦转染到原生质体中，在没有外源添加生长素的情况下可促进体外生长和细胞分裂。
A transferred DNA (T-DNA) tagging vector with the potential to produce dominant mutations was used with cocultured Agrobacterium tumefaciens and protoplasts to tag genes involved in the action of the plant growth substance auxin. Transgenic calli were selected for their ability to grow in the absence of auxin in the culture media. From one experiment, 12 calli that displayed this phenotype were recovered, of which 11 were able to regenerate into plants. In one plant studied in detail, protoplast division in the absence of auxin genetically cosegregated with a single T-DNA insert. A messenger RNA encoded by a 6.4-kilobase sequence of plant genomic DNA rescued from the mutant is overexpressed relative to untransformed plants. The genomic DNA, as well as a cognate complementary DNA, once transfected into protoplasts promote growth and cell division in vitro in the absence of exogenously added auxin.