Influences of protein films on antibacterial or bacteria-repellent surface coatings in a model system using silicon wafers
Influences of protein films on antibacterial or bacteria-repellent surface coatings in a model system using silicon wafers
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DOI：
10.1016/j.biomaterials.2009.05.079
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发表时间：
2009-10-01
期刊：
影响因子：
14
通讯作者：
Schmalz, Gottfried
中科院分区：
文献类型：
--
作者：
Mueller, Rainer;Eidt, Andreas;Schmalz, Gottfried
将确定的化学官能团固定到生物材料表面上，不仅可以优化它们的组织相容性，还可以预防细菌感染。接枝表面与链的聚（乙二醇）（PEG）的结果，在细菌排斥性，而改性与阳离子基团传递它们与杀菌性能。由于生物材料在原位将成为暴露于富含蛋白质的环境中，有必要研究这种类型的化学表面改性的抗菌活性的影响，事先蛋白质吸附。在本研究中，我们将短链PEG和两个含吡啶基团的甲基丙烯酸酯单体，12-甲基丙烯酰氧基-十二烷基溴化吡啶（MDPB）和6-甲基丙烯酰氧基己基氯化吡啶（MHPC）固定到硅片模型表面，以研究先前蛋白质吸附对表面涂层对随后附着的细菌的杀菌活性的影响。吸附量的人血清白蛋白和唾液蛋白被发现是两倍高的阳离子相比，PEG改性的表面。一个类似的趋势，发现附着的格氏链球菌和变形链球菌相同的表面没有事先暴露的蛋白质。然而，大多数附着在阳离子表面的细菌被发现是死的。先前暴露于蛋白质溶液的阳离子表面急剧改变细菌附着，这取决于蛋白质溶液的类型和所用的细菌种类。值得注意的是，发现在吸附蛋白质膜后，吡啶鎓涂层表面的原始杀菌活性大大降低。作为一个结论，我们建议，未来的方法应该结合联合收割机的蛋白质和细菌排斥性能的聚乙二醇涂层的杀菌功能的带电阳离子基团。(C)2009爱思唯尔有限公司保留所有权利。
Immobilization of defined chemical functionalities to biomaterial surfaces is employed to optimize them not only for tissue compatibility but also for prevention of bacterial infection. Grafting surfaces with chains of poly(ethylene glycol) (PEG) results in bacterial repellence whereas modification with cationic groups conveys them with bactericidal properties. Since biomaterials in situ will become exposed to a protein-rich environment, it is necessary to investigate the influence of prior protein adsorption on the antibacterial activity of this type of chemical surface modification. In the present study, we immobilized short-chain PEG and two pyridinium group-containing methacrylate monomers, 12-methacryloyloxy-dodecylpyridinium bromide (MDPB) and 6-methacryloyloxyhexylpyridinium chloride (MHPC), to silicon wafer model surfaces to investigate the influence of prior protein adsorption on the bactericidal activity of the surface coating towards subsequently attached bacteria. Adsorbed amounts of human serum albumin and salivary proteins were found to be two times higher on cationic compared to PEG-modified surfaces. An analogous tendency was found for attachment of Streptococcus gordonii and Streptococcus mutans to the same surfaces without prior protein exposure. However, most bacteria attached to cationic surfaces were found to be dead. Prior exposure of cationic surfaces to protein solutions drastically altered bacterial attachment dependent on the type of protein solution and bacterial species employed. Significantly, the original bactericidal activity of pyridinium-coated surfaces was found greatly reduced upon adsorption of a protein film. As a conclusion we propose that future approaches should combine the protein- and bacteria-repellent properties of PEG-coatings with the bactericidal function of charged cationic groups. (C) 2009 Elsevier Ltd. All rights reserved.