Growth factor induced proliferation, migration, and lumen formation of rat endometrial epithelial cells in vitro.
Growth factor induced proliferation, migration, and lumen formation of rat endometrial epithelial cells in vitro.
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DOI：
10.1262/jrd.2015-158
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发表时间：
2016-06-17
期刊：
影响因子：
--
通讯作者：
Yamauchi N
中科院分区：
文献类型：
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作者：
Islam MR;Yamagami K;Yoshii Y;Yamauchi N
子宫内膜调节对于维持正常的子宫生理是必不可少的，这种调节是由许多生长因子驱动的。本研究研究了表皮生长因子（EGF）和肝细胞生长因子（HGF）对大鼠子宫内膜上皮（REE）细胞的有丝分裂、动性和形态发生的影响。分离培养REE细胞，通过形态学和上皮细胞标志物的表达对其进行表征。MTT实验显示，EGF和HGF可诱导REE细胞增殖。与增殖增加一致，我们发现细胞周期调节因子Cyclin D1在添加EGF和HGF后也上调。Oris细胞迁移实验发现，EGF促进REE细胞迁移。在三维BD Matrigel细胞培养系统中研究了生长因子对REE细胞的形态发生影响，其中这些生长因子也增加了管腔形成的频率。总之，我们发现EGF和HGF对REE细胞有刺激作用，促进增殖、细胞迁移和管腔形成。我们的发现为进一步了解子宫内膜再生及其调控提供了重要的见解。
Endometrial modulation is essential for the preservation of normal uterine physiology, and this modulation is driven by a number of growth factors. The present study investigated the mitogenic, motogenic, and morphogenic effects of epidermal growth factor (EGF) and hepatocyte growth factor (HGF) on rat endometrial epithelial (REE) cells. The REE cells were isolated and cultured and then characterized based on their morphology and their expression of epithelial cell markers. The MTT assay revealed that EGF and HGF induce proliferation of REE cells. Consistent with increased proliferation, we found that the cell cycle regulatory factor Cyclin D1 was also upregulated upon EGF and HGF addition. REE cell migration was prompted by EGF, as observed with the Oris Cell Migration Assay. The morphogenic impact of growth factors on REE cells was studied in a three-dimensional BD Matrigel cell culture system, wherein these growth factors also increased the frequency of lumen formation. In summary, we show that EGF and HGF have a stimulatory effect on REE cells, promoting proliferation, cell migration, and lumen formation. Our findings provide important insights that further the understanding of endometrial regeneration and its regulation.