Effect of a novel palatinose-based liquid balanced formula (MHN-01) on glucose and lipid metabolism in male Sprague-Dawley rats after short- and long-term ingestion
Effect of a novel palatinose-based liquid balanced formula (MHN-01) on glucose and lipid metabolism in male Sprague-Dawley rats after short- and long-term ingestion
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DOI：
10.1016/j.metabol.2004.03.004
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发表时间：
2004-08-01
影响因子：
9.8
通讯作者：
Takeda, E
中科院分区：
文献类型：
--
作者：
Arai, H;Mizuno, A;Takeda, E
餐后高血糖和高胰岛素血症通常出现在肥胖和糖耐量异常的患者中，他们早期胰岛素分泌不足，随后出现延迟的高胰岛素反应。为了解决这个问题，开发了一种名为MHN-01的新型巴氏糖肠溶配方，用于预防餐后高血糖和高胰岛素血症。以标准平衡配方(SBF)为对照，比较MHN-01对SD大鼠糖脂代谢的影响。灌胃等效于0.9g/kg碳水化合物的各方剂后，MHN-01组大鼠外周静脉和门静脉血中的血糖(PG)和胰岛素(IRI)峰值水平均显著低于SBF组。MHN-01组PG和IRI曲线下面积分别为SBF组的58.0%和43.1%，门静脉为65.0%和69.3%。在为期2个月的研究中，与初始水平相比，MHN-01组的血清IRI和外周血甘油三酯水平下降，而SBF组的血清IRI和甘油三酯水平上升。因此，MHN-01组的上述两种水平均显著低于SBF组。此外，与SBF组相比，MHN-01组小鼠腹部脂肪组织和肝组织中的脂肪蓄积量明显减少。MHN-01组胰岛素敏感性高于SBF组，用高胰岛素正血糖钳夹技术评价葡萄糖输注率。因此，与SBF相比，MHN-01可抑制餐后高血糖和高胰岛素血症，减少内脏脂肪堆积，并改善胰岛素敏感性。因此，建议对MHN-01对碳水化合物和脂代谢的影响进行人体研究，以确定MHN-01是否可能是一种治疗和预防胰岛素抵抗的有用功能食品。(C)2004 Elsevier Inc.保留所有权利。
Postprandial hyperglycemia and hyperinsulinemia are often present in obese subjects with glucose intolerance in whom insufficient early phase insulin secretion and subsequent delayed hyperinsulin response are observed. To address this problem, a novel palatinose-based enteral formula designated as MHN-01 was developed for the prevention of postprandial hyperglycemia and hyperinsulinemia. The effects of MHN-01 on carbohydrate and lipid metabolism in Sprague-Dawley (SD) rats were compared with those of the standard balanced formula (SBF). After a bolus intragastric injection of each formula equivalent to 0.9g/kg carbohydrate, the peak levels of plasma glucose (PG) and insulin (IRI) in peripheral and portal veins of the MHN-01 group were significantly lower than those of the SBF group. The areas under the curve of PG and IRI in the MHN-01 group were 58.0% and 43.1% of those in the SBF group in the femoral vein and 65.0% and 69.3% in the portal vein, respectively. In the 2-month study, serum levels of IRI and triglyceride in peripheral blood in the MHN-01 group decreased and those in the SBF group increased compared with initial levels. Consequently, both levels in the MHN-01 group were significantly lower than those in the SBF group. In addition, the amount of accumulated fat in abdominal adipose tissue and liver tissue of the MHN-01 group was markedly reduced in comparison to that of the SBF group. Insulin sensitivity, evaluated as glucose infusion rate using the hyperinsulinemic euglycemic clamp technique, in the MHN-01 group was higher than that in the SBF group. Thus, in comparison to SBF, MHN-01 suppressed postprandial hyperglycemia and hyperinsulinemia, reduced visceral fat accumulation, and improved insulin sensitivity. Therefore, human study on the effects of MHN-01 on carbohydrate and lipid metabolism will be recommended to confirm whether MHN-01 may be a useful functional food for the treatment and prevention of insulin resistance. (C) 2004 Elsevier Inc. All rights reserved.