Immunohistochemical expression of angiogenesis-related markers in oral squamous cell carcinomas with multiple metastatic lymph nodes.
Immunohistochemical expression of angiogenesis-related markers in oral squamous cell carcinomas with multiple metastatic lymph nodes.
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DOI：
10.1309/jd3d-hgcd-gaun-1r0j
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发表时间：
2003-04
影响因子：
3.5
通讯作者：
N. Nikitakis;H. Rivera;M. Lopes;H. Siavash;M. Reynolds;R. Ord;J. Sauk
中科院分区：
文献类型：
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作者：
N. Nikitakis;H. Rivera;M. Lopes;H. Siavash;M. Reynolds;R. Ord;J. Sauk
本研究的目的是评估口腔鳞状细胞癌（SCC）的原发肿瘤和转移淋巴结的组织病理学特征和血管生成相关标志物的表达与多个淋巴结受累的口腔鳞状细胞癌（SCC）相比，没有淋巴结转移。血管生成抑制剂内皮抑制素的蛋白水平，以及相关分子胶原XVIII，胶原结合蛋白（CBP）2/热休克蛋白（HSP）47，和组织蛋白酶L，通过免疫组织化学分析进行了评价。与非转移性病例相比，转移组的原发性肿瘤表现出显着降低的蛋白水平的内皮抑素及其前体胶原XVIII。原发灶与转移灶的阳性淋巴结比较，转移灶中CXVIII和CBP 2/HSP 47的表达明显降低。血管生成对于肿瘤的生长和转移是必不可少的;因此，在口腔鳞状细胞癌中观察到的血管生成相关蛋白的免疫组化表达的差异与多个淋巴结受累可能提供了一个解释这些肿瘤的转移潜力增加。
The aim of this study was to evaluate the histopathologic features and the expression of angiogenesis-related markers in primary tumors and metastatic lymph nodes of oral squamous cell carcinomas (SCCs) with multiple lymph node involvement in comparison with oral SCCs without nodal metastasis. The protein levels of the angiogenesis inhibitor endostatin, as well as those of the related molecules collagen XVIII, collagen-binding protein (CBP) 2/heat shock protein (HSP) 47, and cathepsin L, were evaluated by immunohistochemical analysis. Compared with nonmetastatic cases, primary tumors of the metastatic group exhibited significantly decreased protein levels of endostatin and its precursor collagen XVIII. Comparison between primary tumors and positive nodes of the metastatic cases revealed decreased expression of collagen XVIII and CBP2/HSP47 in metastases. Angiogenesis is essential for tumor growth and metastasis; accordingly, the observed differences in the immunohistochemical expression of angiogenesis-related proteins in oral SCC with multiple lymph node involvement may provide an explanation for the increased metastatic potential of these tumors.