Automated microgradient system for capillary electrochromatography
Automated microgradient system for capillary electrochromatography
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DOI：
10.1016/s0021-9673(02)01806-x
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发表时间：
2003-03-21
影响因子：
4.1
通讯作者：
Welsch, T
中科院分区：
文献类型：
--
作者：
Kahle, V;Vázlerová, M;Welsch, T
本文介绍了一种适用于毛细管电色谱的微机控制梯度洗脱系统。它基于之前描述的液体处理装置，该装置能够在低流体分散和高流体分散的情况下输送液体。用内径为250 μ m的不锈钢针形成的低分散区。用于样品注入，而由管直径的急剧延伸产生的高分散区域用于连续的移动的相梯度产生。在聚丙烯酸酯整体柱上，在梯度条件下，对7种烷基苯酮的同系物进行了电色谱分离。应用S形乙腈梯度（30-70%）。保留时间的重现性高（RSD约0.1%），表明自动梯度操作的准确性。(C)2002 Elsevier Science B. V.保留所有权利。
A microprocessor controlled gradient elution system suitable for capillary electrochromatography has been developed and tested. It is based on a liquid handling device described previously which is capable of liquid transport with both low and high fluid dispersion. The low dispersion region formed by stainless steel needle 250 mum I.D. serves for sample injection, while the high dispersion region, created by steep extension of tube diameter, is used for continuous mobile phase gradient generation. A homologous series of seven alkylphenones was electrochromatographycally separated on a monolithic polyacrylic column under gradient conditions. An S-shaped acetonitrile gradient (30-70%) was applied. A high reproducibility of retention times (RSD about 0.1%) was obtained, indicating accuracy of automated gradient operations. (C) 2002 Elsevier Science B.V. All rights reserved.