CD4+ T Cell Cytokine Gene and Protein Expression in Duodenal Mucosa of Dogs with Inflammatory Bowel Disease
CD4+ T Cell Cytokine Gene and Protein Expression in Duodenal Mucosa of Dogs with Inflammatory Bowel Disease
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DOI：
10.1292/jvms.13-0008
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发表时间：
2014-03-01
影响因子：
1.2
通讯作者：
Takiguchi, Mitsuyoshi
中科院分区：
文献类型：
--
作者：
Ohta, Hiroshi;Takada, Kanae;Takiguchi, Mitsuyoshi
炎症性肠病（IBD）是犬慢性胃肠道体征的常见原因。在人类中，T辅助细胞在IBD的发病机制中起重要作用。相比之下，在犬IBD中没有描述不同T细胞亚群的特异性参与。本研究检测了IBD犬十二指肠黏膜T细胞亚群细胞因子的基因和蛋白表达。采用27只IBD犬和8只对照犬的十二指肠粘膜进行白细胞介素（IL）-17 A、干扰素（IFN）-γ、IL-4和IL-10 mRNA转录的相对定量。采用ELISA法检测15只IBD犬和8只对照犬十二指肠粘膜IL-17 A、IFN-γ和IL-10蛋白水平。各组细胞因子mRNA转录水平差异无统计学意义。各组间IL-17 A、IFN-γ和IL-10蛋白表达水平无显著差异。因此，没有明确的证据表明，不同的Th细胞因子参与犬IBD的发病机制。
Inflammatory bowel disease (IBD) is a common cause of chronic gastrointestinal signs in dogs. In humans, T helper cells have important roles in the pathogenesis of IBD. In contrast, no specific involvement of a distinct T cell subset has been described in canine IBD. The present study evaluated the gene and protein expression of cytokines of T cell subsets in duodenal mucosa from dogs with IBD. Relative quantification of interleukin (IL)-17A, interferon (IFN)-gamma, IL-4 and IL-10 mRNA transcription was performed using duodenal mucosa from 27 IBD dogs and 8 controls. Duodenal mucosal IL-17A, IFN-gamma and IL-10 protein levels were determined by ELISA in 15 IBD dogs and 8 controls. There was no significant difference in each cytokines mRNA transcription level between groups. There was no significant difference in IL-17A, IFN-gamma and IL-10 protein expression levels between groups. Thus, there is no clear evidence for the involvement of distinct Th cytokine in the pathogenesis of canine IBD.