Visualization of synaptic specializations in live C-elegans with synaptic vesicle protein-GFP fusions
Visualization of synaptic specializations in live C-elegans with synaptic vesicle protein-GFP fusions
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DOI：
10.1016/s0165-0270(99)00031-x
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发表时间：
1999-07-01
影响因子：
3
通讯作者：
Nonet, ML
中科院分区：
文献类型：
--
作者：
Nonet, ML
在活体标本中，突触的特化很难在光学显微镜下观察到。为了解决这个问题，将突触囊泡蛋白与绿色荧光蛋白（GFP）融合，并在C.线虫神经元C.观察到秀丽线虫小突触蛋白-GFP和突触回蛋白-GFP融合蛋白靶向突触位点。这种定位允许用光学显微镜观察活体动物的突触特化。在神经元亚群中突触小泡蛋白-GFP融合的受限表达使得个体突触前静脉曲张能够可视化。突触囊泡蛋白的分类、囊泡向末梢的运输以及突触前特化的发展的细胞生物学基础现在更适合于使用这些突触标记进行正向遗传分析。(C)1999 Elsevier Science B. V.保留所有权利。
Synaptic specializations are difficult to visualize at the light microscope level in living preparations. To circumvent this problem, synaptic vesicle proteins were fused to green fluorescent protein (GFP) and expressed in C. elegans neurons. C. elegans synaptobrevin-GFP and synaptogyrin-GFP fusion proteins were observed to target to synaptic sites. This localization allowed the visualization of synaptic specializations in living animals with light microscopy. Restricted expression of synaptobrevin-GFP fusions in subsets of neurons enables the visualization of individual presynaptic varicosities. The cell biology underlying the sorting of synaptic vesicle proteins, trafficking of vesicles to terminals, and the development of presynaptic specializations is now more amenable to forward genetic analysis using these synaptic markers. (C) 1999 Elsevier Science B.V. All rights reserved.