Solubility diagram of the Rhodobacter sphaeroides reaction center as a function of PEG concentration
Solubility diagram of the Rhodobacter sphaeroides reaction center as a function of PEG concentration
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DOI：
10.1016/s0014-5793(96)01446-9
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发表时间：
1997-01-20
期刊：
影响因子：
3.5
通讯作者：
Ducruix, A
中科院分区：
文献类型：
--
作者：
Gaucher, JF;RiesKautt, M;Ducruix, A
为了量化聚乙二醇4000（PEG）对膜蛋白的溶解度的影响，我们通过批量方法在大范围的PEG上结晶了来自球形红细菌Y的光化学反应中心。溶解度图的测量显示溶解度对PEG浓度的半对数依赖性。我们的结果与先前公开的结果的比较[Odahara，T.，Ataka，M.和Katalina，M.（1991）Acta Cryst.D50，639-642]表明另外的1，2，3-庚烷-三醇和/或温度对光化学反应中心溶解度的显著影响。
In order to quantify the effect of polyethylene glycol 4000 (PEG) on the solubility of an integral membrane protein, we have crystallized the photochemical reaction center from Rhodobacter sphaeroides Y by batch method on a large range of PEG, The measurement of the solubility diagram display a semi-logarithmic dependence of solubility versus PEG concentration, Comparison of our results with previously published ones [Odahara, T., Ataka, M. and Katsura, M. (1991) Acta Cryst. D50, 639-642] suggests a notable effect of additional 1,2,3-heptane-triol and/or temperature on photochemical reaction center solubility.