Augmented inhibitory effect of superoxide dismutase on superoxide anion release from macrophages by chemical modification with polysaccharide and attenuation effects on radiation-induced inflammatory cytokine expression in vitro
Augmented inhibitory effect of superoxide dismutase on superoxide anion release from macrophages by chemical modification with polysaccharide and attenuation effects on radiation-induced inflammatory cytokine expression in vitro
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多糖化学修饰增强超氧化物歧化酶对巨噬细胞释放超氧阴离子的抑制作用，并减弱体外辐射诱导的炎症细胞因子表达的作用
DOI：
10.1080/10611860802669249
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发表时间：
2009-04
影响因子：
4.5
通讯作者：
Wang, Fengshan
中科院分区：
文献类型：
--
作者：
Liu, Chunhui;Hu, Likuan;Wang, Yonggang;Liu, Jinfeng;Teng, Li;Liu, Hong;Wang, Fengshan
为了提高超氧化物歧化酶（SOD）抑制活性氧（ROS）介导的损伤的能力，设计并制备了N,N,N-三甲基氯化壳聚糖（TMC）和肝素化学修饰的SOD衍生物、阳离子化SOD（TMC-SOD）和阴离子化SOD（肝素-SOD）。本研究体外研究了TMC-SOD和肝素-SOD对巨噬细胞释放超氧阴离子的抑制作用。 TMC-SOD和肝素-SOD均对巨噬细胞释放超氧阴离子表现出优异的抑制作用，且TMC-SOD的效果超过天然SOD和肝素-SOD。还评估了 TMC-SOD 和肝素-SOD 对体外炎症细胞因子表达的影响。结果表明，TMC-SOD和肝素-SOD均能显着降低受辐射的3T3成纤维细胞表达的转化生长因子-β1（TGF-β1）和白细胞介素-1β（IL-1β）的水平。这些结果表明，阳离子多糖或阴离子多糖SOD衍生物可能可用于预防和治疗ROS介导的炎症性疾病。这项研究还表明，SOD 的化学修饰，尤其是阳离子化，大大增强了 SOD 的细胞内递送，从而对 ROS 介导的损伤产生显着的保护作用。
To improve the ability of superoxide dismutase (SOD) to suppress reactive oxygen species (ROS)-mediated injury, chemically modified derivatives of SOD with N,N,N-trimethyl chitosan chloride (TMC) and heparin, cationized SOD (TMC-SOD), and anionized SOD (heparin-SOD) were designed and prepared. In this study, the inhibitory effect of TMC-SOD and heparin-SOD on superoxide anion release from macrophages was studied in vitro. Both TMC-SOD and heparin-SOD exhibited excellent inhibitory effects on superoxide anion release from macrophages, and the effects of TMC-SOD surpassed those of native SOD and heparin-SOD. The effects of TMC-SOD and heparin-SOD on inflammatory cytokine expression in vitro were also evaluated. The results showed that both TMC-SOD and heparin-SOD could significantly lower the levels of transforming growth factor-β1 (TGF-β1) and interleukine-1β (IL-1β) expressed by irradiated 3T3 fibroblasts. These results demonstrated that cationic polysaccharide or anionic polysaccharide SOD derivatives might be useful in the prevention and treatment of ROS-mediated inflammatory diseases. This study also demonstrated that chemical modification of SOD, especially cationization, greatly enhanced SOD’s intracellular delivery and, consequently, produced a significant protective effect against ROS-mediated injury.
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DOI：
--
发表时间：
2001-09
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Y. Yabe;Naoki Kobayashi;Tsuyoshi Nishihashi;R. Takahashi;M. Nishikawa;Y. Takakura;M. Hashida
通讯作者：
Y. Yabe;Naoki Kobayashi;Tsuyoshi Nishihashi;R. Takahashi;M. Nishikawa;Y. Takakura;M. Hashida
影响因子：
5.6
作者：
CASTELLOT, JJ;WONG, K;KARNOVSKY, MJ
通讯作者：
KARNOVSKY, MJ
DOI：
10.1016/s0939-6411(03)00151-6
发表时间：
2004-01-01
影响因子：
4.9
作者：
Polnok, A;Borchard, G;Junginger, HE
通讯作者：
Junginger, HE
DOI：
10.1172/jci111946
发表时间：
1985-07
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
J. Schalkwijk;Wim;B. V. den;Berg;Levinus B. A. van de;Putte;Leo;A. B. Joosten;Liduine;van den Bersselaar
通讯作者：
J. Schalkwijk;Wim;B. V. den;Berg;Levinus B. A. van de;Putte;Leo;A. B. Joosten;Liduine;van den Bersselaar
DOI：
10.1172/jci111604
发表时间：
1984-11
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
W. B. van den Berg;L. van de Putte;W. A. Zwarts;L. Joosten
通讯作者：
W. B. van den Berg;L. van de Putte;W. A. Zwarts;L. Joosten