A genomewide oscillation in transcription gates DNA replication and cell cycle
A genomewide oscillation in transcription gates DNA replication and cell cycle
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DOI：
10.1073/pnas.0306490101
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发表时间：
2004-02-03
影响因子：
11.1
通讯作者：
Murray, DB
中科院分区：
文献类型：
--
作者：
Klevecz, RR;Bolen, J;Murray, DB
酵母连续同步培养系统的微阵列分析显示转录存在全基因组振荡。在这个大约 40 分钟的呼吸和还原循环中，转录水平的最大值出现在三个几乎相等的间隔处。两个时间簇（5,329 个中的 4,679 个）在周期的还原期表达最大，而第三个簇 (650) 在呼吸期表达最大。转录在功能上被组织成氧化还原态超簇，其中已知对呼吸或还原很重要的基因在循环的相反阶段合成。转录周期以 40 分钟的间隔在恒定比例的群体中控制 DNA 复制的同步爆发。将 DNA 合成限制在循环的还原阶段可能是减少复制过程中 DNA 氧化损伤的重要进化机制。
Microarray analysis from a yeast continuous synchrony culture system shows a genomewide oscillation in transcription. Maximums in transcript levels occur at three nearly equally spaced intervals in this approximate to40-min cycle of respiration and reduction. Two temporal clusters (4,679 of 5,329) are maximally expressed during the reductive phase of the cycle, whereas a third cluster (650) is maximally expressed during the respiratory phase. Transcription is organized functionally into redox-state superclusters with genes known to be important in respiration or reduction being synthesized in opposite phases of the cycle. The transcriptional cycle gates synchronous bursts in DNA replication in a constant fraction of the population at 40-min intervals. Restriction of DNA synthesis to the reductive phase of the cycle may be an evolutionarily important mechanism for reducing oxidative damage to DNA during replication.