Age-dependent poliovirus replication in the mouse central nervous system is determined by internal ribosome entry site-mediated translation.
Age-dependent poliovirus replication in the mouse central nervous system is determined by internal ribosome entry site-mediated translation.
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小鼠中枢神经系统中年龄依赖性脊髓灰质炎病毒的复制是由内部核糖体进入位点介导的翻译决定的。
DOI：
10.1128/jvi.80.6.2589-2595.2006
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Racaniello,VincentR
中科院分区：
文献类型：
--
作者：
Kauder,Steven;Kan,Sherry;Racaniello,VincentR
Mouse cells are not permissive for the replication of human rhinovirus type 2 (HRV2). To determine the role of the HRV2 internal ribosome entry site (IRES) in determining species specificity, a recombinant poliovirus (P1/HRV2) was constructed by substituting the poliovirus IRES with the IRES from HRV2. This recombinant virus replicated in all human and murine cell lines examined, demonstrating that the HRV2 IRES does not limit viral replication in transformed murine cells. P1/HRV2 replicated in the brain and spinal cord in neonatal but not adult mice transgenic for the poliovirus receptor, CD155. Passage of P1/HRV2 in mice led to selection of a virus that caused paralysis in neonatal mice. To determine the relationship between HRV2 IRES-mediated translation and replication of P1/HRV2 in mice, recombinant human adenoviruses were used to express bicistronic mRNAs in murine organs. The results demonstrate that the HRV2 IRES mediates translation in organs of neonatal but not adult mice. These findings show that HRV2 IRES-mediated translation is a determinant of virus replication in the murine brain and spinal cord and suggest that the IRES determines the species specificity of HRV2 infection.
