Detection of somatic DNA recombination in the transgenic mouse brain.
Detection of somatic DNA recombination in the transgenic mouse brain.
复制标题
转基因小鼠脑中体细胞DNA重组的检测。
DOI：
10.1126/science.1925563
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Sakano,H
中科院分区：
文献类型：
--
作者：
Matsuoka,M;Nagawa,F;Okazaki,K;Kingsbury,L;Yoshida,K;Müller,U;Larue,DT;Winer,JA;Sakano,H
用含有细菌β-半乳糖苷酶基因（lacZ）的DNA构建体研究转基因小鼠脑中的体细胞DNA重组。成年脑的变性阳性区域被β-半乳糖苷酶的底物X-gal染成蓝色。蓝色细胞在出生后不久就出现了，并继续出现在出生后的组织中。与大脑的运动区域相比，染色在感觉区域中突出，并且存在于神经系统的70多个离散区域中。DNA重排的可能性进行了讨论相对于中枢神经系统的发展。
A DNA construct containing the bacterial β-galactosidase gene (lacZ) was used to study somatic DNA recombination in the transgenic mouse brain. Recombination-positive areas of the adult brain were stained blue with X-gal, a substrate of β-galactosidase. Blue-colored cells appeared soon after birth, and continued to emerge in postnatal tissue. Staining was prominent in sensory as opposed to motor regions of the brain, and was present in more than 70 discrete areas of the nervous system. The possibility of DNA rearrangement is discussed with respect to the development of the central nervous system.