Applications and Prospects of CRISPR/Cas9-Mediated Base Editing in Plant Breeding.
Applications and Prospects of CRISPR/Cas9-Mediated Base Editing in Plant Breeding.
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DOI：
10.3390/cimb45020059
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发表时间：
2023-01-19
影响因子：
3.1
通讯作者：
Li, Juan
中科院分区：
文献类型：
--
作者：
Li, Yizhen;Liang, Jing;Deng, Bufang;Jiang, Yingli;Zhu, Jingyan;Chen, Like;Li, Min;Li, Juan
成簇的规则间隔短回文重复序列（CRISPR）/相关蛋白9系统（Cas9）已被详细用于优化种质资源的多个方面。然而，大规模的基因组研究表明，作物中的新变异归因于单核苷酸多态性（SNP）。因此，将单个碱基替换到植物基因组中可以产生期望的性状。通过CRISPR/Cas9技术进行的基因编辑通常会导致插入-缺失（indel）。碱基编辑允许在不存在双链断裂（DSB）和供体修复模板（DRT）的情况下在基因组中进行精确的单核苷酸改变。因此，生物工程为基因组编辑提供了一种新的思路，基础编辑技术目前正被用于编辑许多不同生物体的基因组。随着传统育种技术和现代分子育种技术的相互补充，各种基因组编辑技术应运而生。如何实现BE应用的更大潜力是我们需要考虑的问题。在这里，我们解释各种基本编辑，如CBE，ABE和CGBE。此外，还总结了碱基编辑技术在农业中的最新应用，包括作物产量、品质、病害和除草剂抗性。最后，提出了基础编辑技术面临的挑战和未来的展望。本文旨在对BE在作物育种中的应用作一综述，以进一步提高BE的利用价值。
The clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein 9 system (Cas9) has been used at length to optimize multiple aspects of germplasm resources. However, large-scale genomic research has indicated that novel variations in crop plants are attributed to single-nucleotide polymorphisms (SNPs). Therefore, substituting single bases into a plant genome may produce desirable traits. Gene editing by CRISPR/Cas9 techniques frequently results in insertions–deletions (indels). Base editing allows precise single-nucleotide changes in the genome in the absence of double-strand breaks (DSBs) and donor repair templates (DRTs). Therefore, BEs have provided a new way of thinking about genome editing, and base editing techniques are currently being utilized to edit the genomes of many different organisms. As traditional breeding techniques and modern molecular breeding technologies complement each other, various genome editing technologies have emerged. How to realize the greater potential of BE applications is the question we need to consider. Here, we explain various base editings such as CBEs, ABEs, and CGBEs. In addition, the latest applications of base editing technologies in agriculture are summarized, including crop yield, quality, disease, and herbicide resistance. Finally, the challenges and future prospects of base editing technologies are presented. The aim is to provide a comprehensive overview of the application of BE in crop breeding to further improve BE and make the most of its value.
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影响因子：
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