Illuminating the oral microbiome and its host interactions: tools and approaches for molecular ecological studies.
Illuminating the oral microbiome and its host interactions: tools and approaches for molecular ecological studies.
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阐明口腔微生物组及其宿主相互作用：分子生态研究的工具和方法。
DOI：
10.1093/femsre/fuac052
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发表时间：
2023
影响因子：
11.3
通讯作者：
Merritt,Justin
中科院分区：
文献类型：
--
作者：
Kreth,Jens;Merritt,Justin
对龋齿和牙周炎等口腔疾病的更全面的了解取决于对导致疾病发展的微生物生态过程的深入了解。通过这篇综述，我们全面概述了相关的分子生态学技术，这些技术在当前对人类口腔生物膜发育、种间相互作用和微生物组生物地理学的理解中发挥了关键作用。主要重点是口腔微生物学文献中提供的相关技术和示例。然而，大多数（如果不是全部）所描述的技术应该很容易适用于体内其他粘膜部位的微生物组的研究。因此，这篇综述旨在为微生物组研究人员提供参考指南，因为他们不可避免地要转向临床观察到的许多重要表型的分子机制研究。
A more comprehensive understanding of oral diseases like caries and periodontitis is dependent on an intimate understanding of the microbial ecological processes that are responsible for disease development. With this review, we provide a comprehensive overview of relevant molecular ecology techniques that have played critical roles in the current understanding of human oral biofilm development, interspecies interactions, and microbiome biogeography. The primary focus is on relevant technologies and examples available in the oral microbiology literature. However, most, if not all, of the described technologies should be readily adaptable for studies of microbiomes from other mucosal sites in the body. Therefore, this review is intended to serve as a reference guide used by microbiome researchers as they inevitably transition into molecular mechanistic studies of the many significant phenotypes observed clinically.