Low-level laser therapy decreases levels of lung neutrophils anti-apoptotic factors by a NF-κB dependent mechanism
Low-level laser therapy decreases levels of lung neutrophils anti-apoptotic factors by a NF-κB dependent mechanism
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DOI：
10.1016/j.intimp.2007.12.007
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发表时间：
2008-04-01
影响因子：
5.6
通讯作者：
Pacheco-Soares, C.
中科院分区：
文献类型：
--
作者：
Aimbire, F.;Santos, F. V.;Pacheco-Soares, C.
背景和目的：低强度激光治疗（LLLT）是一种已知的炎症过程调节剂。本文研究了660 nm半导体激光对脂多糖（LPS）诱导的肺部炎症中性粒细胞抗凋亡因子mRNA水平的影响。研究设计/方法：将小鼠分为8组（每组n=7），并以7.5 J/cm（2）的能量剂量进行照射。采用真实的时间-PCR（RT-PCR）检测中性粒细胞Bcl-xL和A1 mRNA的表达。在LPS暴露时间后照射动物。结果如下：LLLT和NF-κ B核转位抑制剂（BMS 205820）可降低LPS诱导炎症小鼠肺中性粒细胞中Bcl-xL和A1 mRNA的mRNA水平。结论：LLLT通过NF-κ B B参与的作用机制降低LPS致炎小鼠肺巨噬细胞中抗凋亡因子的水平。(c)2008年由Elsevier B. V.出版。
Background and objective: Low-level laser therapy (LLLT) is a known modulator of inflammatory process. Herein we studied the effect of 660 nm diode laser on mRNA levels of neutrophils anti-apoptotic factors in lipopolysaccharide (LPS)-induced lung inflammation. Study design/ methodology: Mice were divided into 8 groups (n=7 for each group) and irradiated with energy dosage of 7.5 j/cm(2). The Bcl-xL and A1 mRNA levels in neutrophils were evaluated by Real Time-PCR (RT-PCR). The animals were irradiated after exposure time of LPS. Results: LLLT and an inhibitor of NF-kappa B nuclear translocation (BMS 205820) attenuated the mRNA levels of Bcl-xL and A1 mRNA in lung neutrophils obtained from mice subjected to LPS-induced inflammation. Conclusion: LLLT reduced the levels of anti-apoptotic factors in LPS inflamed mice lung neutro-phils by an action mechanism in which the NF-kappa B seems to be involved. (c) 2008 Published by Elsevier B.V.