Cultivation-independent examination of horizontal transfer and host range of an IncP-1 plasmid among gram-positive and gram-negative bacteria indigenous to the barley rhizosphere
Cultivation-independent examination of horizontal transfer and host range of an IncP-1 plasmid among gram-positive and gram-negative bacteria indigenous to the barley rhizosphere
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DOI：
10.1128/aem.00013-06
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发表时间：
2006-10-01
影响因子：
4.4
通讯作者：
Sorensen, Soren J.
中科院分区：
文献类型：
--
作者：
Musovic, Sanin;Oregaard, Gunnar;Sorensen, Soren J.
研究了IncP-1质粒（pKJK 10）在大麦根际土著细菌中的宿主范围和转移频率。一种新的流式细胞术为基础的培养无关的计数和随后的16 S rRNA基因分类的transconjugants分选方法。土著transconjugant根际细菌收集荧光激活细胞分选和鉴定的克隆和测序的16 S rRNA基因从分选的细胞。pKJK 10质粒的宿主范围非常广泛，因为它不仅包括属于变形菌门的α、β和γ亚类的细菌，而且包括节杆菌属，革兰氏阳性的放线菌。从恶臭假单胞菌供体到固有细菌的转移频率（每个供体的转移接合物）为7.03 × 10（-2）+/- 3.84 × 10（-2）。这是第一次直接记录革兰氏阴性供体和革兰氏阳性受体细菌原位接合转移。
The host range and transfer frequency of an IncP-1 plasmid (pKJK10) among indigenous bacteria in the barley rhizosphere was investigated. A new flow cytometry-based cultivation-independent method for enumeration and sorting of transconjugants for subsequent 16S rRNA gene classification was used. Indigenous transconjugant rhizosphere bacteria were collected by fluorescence-activated cell sorting and identified by cloning and sequencing of 16S rRNA genes from the sorted cells. The host range of the pKJK10 plasmid was exceptionally broad, as it included not only bacteria belonging to the alpha, beta, and gamma subclasses of the Proteobacteria, but also Arthrobacter sp., a gram-positive member of the Actinobacteria. The transfer frequency (transconjugants per donor) from the Pseudomonas putida donor to the indigenous bacteria was 7.03 x 10(-2) +/- 3.84 X 10(-2). This is the first direct documentation of conjugal transfer between gram-negative donor and gram-positive recipient bacteria in situ.