Uterine Microbiota Progression from Calving until Establishment of Metritis in Dairy Cows
Uterine Microbiota Progression from Calving until Establishment of Metritis in Dairy Cows
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DOI：
10.1128/aem.01753-15
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发表时间：
2015-09-01
影响因子：
4.4
通讯作者：
Galvao, Klibs N.
中科院分区：
文献类型：
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作者：
Jeon, Soo Jin;Vieira-Neto, Achilles;Galvao, Klibs N.
本研究的目的是评估从产犊到子宫炎建立的子宫微生物群的进展。在产后0、2和6 +/-2天（dpp）从12头子宫炎和12头健康奶牛收集的子宫拭子（n = 72）用于在Illumina MiSeq平台上进行16 S rRNA基因的宏基因组测序。热图显示子宫微生物群在产犊时建立。微生物群从0到6 +/- 2 dpp迅速变化，变形菌门丰度降低，拟杆菌门和梭菌门丰度增加，这在子宫炎奶牛中占主导地位。子宫微生物群组成是相同的;但是，可以使用0、2和6 +/- 2 dpp时细菌属的相对丰度来区分子宫炎和健康奶牛。拟杆菌属是与子宫炎相关的主要属，因为它是唯一在患有子宫炎的奶牛中表现出显著更高丰度的属。随着拟杆菌生物丰度的增加，子宫分泌物评分（衡量子宫健康的指标）恶化。梭杆菌也是与子宫炎相关的重要属，因为梭杆菌丰度随着拟杆菌丰度增加而增加，并且随着丰度增加，子宫分泌物评分恶化。与子宫分泌物评分的相关性以及与拟杆菌属或梭杆菌属的相关性表明，其他细菌，如Helcoccocus、Filifactor和Porphyromonas，也与子宫炎相关。还有一些与子宫健康有关的细菌，如“Blochmannia”、埃希氏菌、Sneathia和Pedobacter。
The objective of this study was to evaluate the progression of the uterine microbiota from calving until establishment of metritis. Uterine swabs (n = 72) collected at 0, 2, and 6 +/- 2 days postpartum (dpp) from 12 metritic and 12 healthy cows were used for metagenomic sequencing of the 16S rRNA gene on the Illumina MiSeq platform. A heat map showed that uterine microbiota was established at calving. The microbiota changed rapidly from 0 to 6 +/- 2 dpp, with a decrease in the abundance of Proteobacteria and an increase in the abundance of Bacteroidetes and Fusobacteria, which were dominant in metritic cows. Uterine microbiota composition was shared; however, metritic and healthy cows could be discriminated using relative abundance of bacterial genera at 0, 2, and 6 +/- 2 dpp. Bacteroides was the main genus associated with metritis because it was the only genus that showed significantly greater abundance in cows with metritis. As the abundance of Bacteroides organisms increased, the uterine discharge score, a measure of uterine health, worsened. Fusobacterium was also an important genus associated with metritis because Fusobacterium abundance increased as Bacteroides abundance increased and the uterine discharge score worsened as the abundance increased. The correlation with uterine discharge score and the correlation with Bacteroides or Fusobacterium showed that other bacteria, such as Helcoccocus, Filifactor, and Porphyromonas, were also associated with metritis. There were also bacteria associated with uterine health, such as "Candidatus Blochmannia," Escherichia, Sneathia, and Pedobacter.