Preservative Effects of Trehalose on Rat Muscle Flaps
Preservative Effects of Trehalose on Rat Muscle Flaps
复制标题
海藻糖对大鼠肌瓣的防腐作用
DOI：
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发表时间：
1996
影响因子：
1.5
通讯作者：
Y. Nishimura
中科院分区：
文献类型：
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作者：
C. W. Zhan;Yoshihisa Suzuki;A. Kitahara;H. Wada;Y. Nishimura
本实验研究海藻糖对大鼠股薄肌皮瓣保存的影响。将肌瓣保存在4°C Euro-Collins溶液或4°C海藻糖-Euro-Collins溶液中6、12、24、48、72和96小时，然后使用显微外科技术重新移植到相同的大鼠体内。保存后即刻和再植后1周，海藻糖-Euro-Collins溶液保存的皮瓣中三磷酸腺苷和磷酸肌酸的水平显着高于Euro-Collins溶液保存的皮瓣。 4℃欧柯林斯溶液组再植后1周，保存12小时后可见局灶性坏死、透明变性、成纤维细胞增殖，保存48小时后无皮瓣成活。 4°C海藻糖-Euro-Collins组仅在保存24小时后才出现局灶性坏死、透明变性和成纤维细胞增殖，而在保存96小时后，8个皮瓣中有2个存活。这些发现表明海藻糖对于保存肌瓣是有效的。
In this experiment, the effect of trehalose on the preservation of rat gracilis muscle flaps was studied. The muscle flaps were preserved in 4° C Euro-Collins solution or 4° C trehalose-Euro-Collins solution for 6, 12, 24, 48, 72, and 96 hours, and then replanted in the same rats using microsurgical techniques. Immediately after preservation and 1 week after replantation, the levels of adenosine triphosphate and creatine phosphate in flaps preserved in the trehalose-Euro-Collins solution were significantly higher than those in flaps preserved in the Euro-Collins solution. In the 4° C Euro-Collins solution group 1 week after replantation, focal necrosis, hyaline degeneration, and proliferation of fibroblasts were seen after 12 hours of preservation, and, after 48 hours of preservation, there were no surviving flaps. In the 4° C trehalose-Euro-Collins group, focal necrosis, hyaline degeneration, and proliferation of fibroblasts were seen only after 24 hours of preservation, and, after 96 hours of preservation, two of eight flaps survived. These findings indicate that trehalose was effective in the preservation of muscle flaps.