Synthesis of novel phosphoramidite reagents for the attachment of antisense oligonucleotides to various regions of the benzophenanthridine ring system.
Synthesis of novel phosphoramidite reagents for the attachment of antisense oligonucleotides to various regions of the benzophenanthridine ring system.
复制标题
合成新型亚磷酰胺试剂，用于将反义寡核苷酸连接到苯并菲啶环系统的各个区域。
DOI：
10.1021/bc00034a018
复制
发表时间：
1995
影响因子：
4.7
通讯作者：
Cushman,M
中科院分区：
文献类型：
--
作者：
Chen,JK;Weith,HL;Grewal,RS;Wang,G;Cushman,M
合成了四种苯并菲并亚磷酰胺试剂，它们之间的连接链连接在苯并菲并菲环系的C-2、C-6、C-9和C-12上。这些苯并菲并[亚磷酰胺]应该被证明在合成反义寡核苷酸-嵌入物方面是有用的，其中连接链连接到苯并[菲]并[1]的不同区域。用一种新的苯并菲并亚胺试剂制备了反义寡核苷酸插层偶联物，其中寡核苷酸TCAGTGGTp通过连接链连接到苯并菲并菲的C-2羟基上。
Four benzophenanthridine phosphoramidite reagents havebeen preparedin which the linker chain between the benzophenanthridine and the phosphoramidite moiety is attached to C-2, C-6, C-9, and C-12 of the benzophenanthridine ring system. These benzophenanthridine phosphoramidites should prove to be useful in the syntheses of antisense oligonucleotide-intercalator conjugates in which the linker chain is attached tovarious regions of the benzophenanthridine intercalator. One of the new benzophenanthridine phosphoramidite reagents was used to prepare an antisense oligonucleotideintercalator conjugate in which the oligonucleotide TCAGTGGTp was connected at its 5'-hydroxyl group through a linker chain to the C-2 hydroxyl group of a benzophenanthridine.