Understanding superinfection exclusion by complex populations of Citrus tristeza virus
Understanding superinfection exclusion by complex populations of Citrus tristeza virus
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DOI：
10.1016/j.virol.2016.10.001
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发表时间：
2016-12-01
期刊：
影响因子：
3.7
通讯作者：
Folimonova, Svetlana Y.
中科院分区：
文献类型：
--
作者：
Bergua, Maria;Kang, Sung-Hwan;Folimonova, Svetlana Y.
重复感染排除（SIE）是一种现象，其中原发性病毒感染限制了相同或密切相关病毒的继发性感染。以前，我们表明，SIE柑橘衰退病毒（CTV）只发生在相同的病毒基因型的分离株。然而，这项工作是使用单一的基因型包含分离株，而大多数田间柑橘树窝藏复杂的人口组成的不同病毒基因型。在这里，我们研究了SIE同时感染几个CTV基因型的植物。实验表明，排除由CTV变异体的继发感染是由同一基因型的另一种变异体在初级群体中的存在触发的，即使在其低水平积累的条件下，并且不受额外的异源基因型的共同出现的影响。同样的规则似乎是有效的，当SIE的混合群体进行了测试，在一系列不同的柑橘品种。
Superinfection exclusion (SIE) is a phenomenon in which a primary viral infection restricts a secondary infection with the same or closely related virus. Previously we showed that SIE by Citrus tristeza virus (CTV) occurs only between isolates of the same virus genotype. This work, however, was done using single genotype containing isolates, while most field citrus trees harbor complex populations composed of different virus genotypes. Here we examined SIE in plants simultaneously infected with several CTV genotypes. The experiments showed that exclusion of a secondary infection by a CTV variant was triggered by the presence of another variant of the same genotype in the primary population, even under the conditions of its low-level accumulation, and was not affected by co-occurrence of additional heterologous genotypes. The same rule appeared to be in effect when SIE by mixed populations was tested in a series of different citrus varieties.