Bacteriophages from Arsenic-Resistant Bacteria Transduced Resistance Genes, which Changed Arsenic Speciation and Increased Soil Toxicity
Bacteriophages from Arsenic-Resistant Bacteria Transduced Resistance Genes, which Changed Arsenic Speciation and Increased Soil Toxicity
复制标题
抗砷细菌的噬菌体转导抗性基因，改变砷形态并增加土壤毒性
DOI：
10.1021/acs.estlett.9b00600
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发表时间：
2019
影响因子：
10.9
通讯作者：
Alvarez Pedro J.
中科院分区：
文献类型：
--
作者：
Tang Xiang;Yu Pingfeng;Tang Lin;Zhou Man;Fan Changzheng;Lu Yue;Mathieu Jacques;Xiong Weiping;Alvarez Pedro J.
溶源性噬菌体被认为是参与环境污染物生物转化的细菌基因的转移载体。然而，它们在砷污染环境中的作用在很大程度上是没有文献记载的。在这两个污染场地的土壤样品中，用丝裂霉素C化学诱导了溶原噬菌体，并在这些噬菌体基因组中检测到了抗砷基因arsC(编码As(V)还原到可排泄(通过外排泵)但毒性更高)和arsM(编码As(III)甲基化)。这些基因的相对丰度(每噬菌体颗粒)与相应的本地土壤细菌群落的相对丰度(每16SRRNA的抗性基因)呈正相关，arsC和arsM的R2分别为0.974和0.761。用100 mg/kg砷土壤进行的微宇宙研究表明，与未改良土壤相比，噬菌体(修正后为每克土壤5.0×107个噬菌体)可使arsC和arsM的繁殖分别增加122倍和575倍。这使As(III)浓度增加了4.3...
Lysogenic phages are known to serve as transfer vectors for bacterial genes involved in biotransformation of various environmental pollutants. However, their role in arsenic-contaminated environments is largely undocumented. Here, lysogenic phages were chemically induced (with mitomycin C) in soil samples from two contaminated sites, and arsenic resistance genes arsC (coding for As(V) reduction to excretable (via efflux pumps) but more toxic As(III)) and arsM (coding for As(III) methylation) were detected in these phage genomes. The relative abundance of these genes (per phage particle) was positively correlated with that in the corresponding indigenous soil bacterial communities (resistance gene per 16S rRNA), with R2 = 0.974 for arsC and 0.761 for arsM. Microcosm studies with 100 mg/kg of arsenic soil showed that phages (amended at 5.0 × 107 phages per gram soil) enhanced the propagation of arsC by 122-fold and arsM by 575-fold, relative to unamended soil. This increased the As(III) concentration by 4.3...