Borrelia burgdorferi and Escherichia coli lipopolysaccharides induce nitric oxide and interleukin-6 production in cultured rat brain cells.
Borrelia burgdorferi and Escherichia coli lipopolysaccharides induce nitric oxide and interleukin-6 production in cultured rat brain cells.
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伯氏疏螺旋体和大肠杆菌脂多糖诱导培养的大鼠脑细胞中一氧化氮和白细胞介素 6 的产生。
DOI：
10.1093/infdis/169.5.1014
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Reichlin,S
中科院分区：
文献类型：
--
作者：
Tatro,JB;Romero,LI;Beasley,D;Steere,AC;Reichlin,S
Borrelia burgdorferi, the spirochetal agent of Lyme disease, infects the central nervous system (CNS), but the factors that mediate inflammation and neurologic dysfunction are not known. SonicatedB. burgdorferistimulated in a concentration-dependent manner the production of nitric oxide (NO) in glial-enriched primary cultures of neonatal rat brain cells via induction of NO synthase activity. Lipopolysaccharide (LPS) ofEscherichia colialso stimulated nitrite accumulation in a concentration-dependent manner. Stimulation of NO production byB. burgdorferisonicate andE. coliLPS was associated with increased levels of mRNA coding for the cytokineinducible form of NO synthase.B. burgdorferisonicate also stimulated release of interleukin-6, with a concentration-response relationship similar to that for its stimulation of nitrite production, as didE. coliLPS. A competitive antagonist ofE. coliLPS,Rhodopseudomonas sphaeroideslipid A, inhibited LPS-induced stimulation of NO synthase activity but markedly potentiated that ofB. burgdorferi, indicating that the initial triggering mechanism ofB. burgdorferiis distinct from that ofE. coliLPS. Induction of NO synthase by bacterial agents within the brain may represent a common pathway of CNS inflammation and neurotoxicity.
