Transforming growth factor β (TGF-β) levels in the conditioned media of human bone cells:: Relationship to donor age, bone volume, and concentration of TGF-β in human bone matrix in vivo
Transforming growth factor β (TGF-β) levels in the conditioned media of human bone cells:: Relationship to donor age, bone volume, and concentration of TGF-β in human bone matrix in vivo
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DOI：
10.1016/s8756-3282(99)00082-4
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发表时间：
1999-06-01
期刊：
影响因子：
4.1
通讯作者：
Pfeilschifter, J
中科院分区：
文献类型：
--
作者：
Bismar, H;Klöppinger, T;Pfeilschifter, J
转化生长因子-β（TGF-β）被认为在人类骨重建中起重要作用。在本研究中，我们研究了112名年龄在28-79岁的女性髂嵴的原代骨细胞培养物中TGF-β水平的组成性差异。TGF-β 1是条件培养基中的主要TGF-β同种型，如通过在水貂肺细胞生物测定中用针对TGF-β 1-3的特异性抗体中和TGF-β活性以及通过酶联免疫测定（ELISA）所确定的，条件培养基中的TGF-β 1水平不随供体年龄而变化，体外TGF-β水平与体内基质相关TGF-β浓度之间缺乏相关性。在整个研究人群中，TGF-β 1水平与局部骨小梁体积无关（r =+0.15，p = 0.16，n = 89）。TGF-β 1水平和骨体积之间的显著相关性存在于绝经前妇女中（r =+0.39，p = 0.02，n = 33），但主要由两个具有最高TGF-β浓度的样本解释。总之，我们的数据表明，TGF-β 1是体外人骨细胞产生的主要TGF-β亚型，骨细胞组成性分泌TGF-β不随年龄变化。TGF-β分泌的组成性差异是否可能是人类骨量的决定因素仍有待于进一步研究。(Bone 24：565-569; 1999）（C）1999，Elsevier Science Inc. All rights reserved.
Transforming growth factor-beta (TGF-beta) is thought to play an important role in human bone remodeling. In the present study, we examined constitutive differences in TGF-beta levels in primary bone cell cultures from the iliac crest of 112 women, aged 28-79 years. TGF-beta 1 was the major TGF-beta isoform in the conditioned media, as determined by neutralizing TGF-beta activity with specific antibodies against TGF-beta 1-3 in the mink lung cell bioassay, and by enzyme-linked immunoassay (ELISA), TGF-beta 1 levels in the conditioned media did not change with donor age, There was a lack of association between TGF-beta levels in vitro and the concentration of matrix-associated TGF-beta in vivo. TGF-beta 1 levels failed to be associated with the local trabecular bone volume in the complete study population (r = +0.15, p = 0.16, n = 89). A significant association between TGF-beta 1 levels and bone volume was present in premenopausal women (r = +0.39, p = 0.02, n = 33), but was largely accounted for by the two samples with the highest TGF-beta concentrations. In conclusion, our data suggest that TGF-beta 1 is the major TGF-beta isoform produced by human bone cells in vitro, and that the constitutive secretion of TGF-beta by bone cells does not change with age. Whether constitutive differences in TGF-beta secretion may be a determinant of human bone mass remains to be clarified in further studies. (Bone 24:565-569; 1999) (C) 1999 by Elsevier Science Inc. All rights reserved.