THE ALPHA-5-GENE PRODUCT ASSEMBLES WITH MULTIPLE ACETYLCHOLINE-RECEPTOR SUBUNITS TO FORM DISTINCTIVE RECEPTOR SUBTYPES IN BRAIN
THE ALPHA-5-GENE PRODUCT ASSEMBLES WITH MULTIPLE ACETYLCHOLINE-RECEPTOR SUBUNITS TO FORM DISTINCTIVE RECEPTOR SUBTYPES IN BRAIN
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DOI：
10.1016/0896-6273(92)90031-8
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发表时间：
1992-10-01
期刊：
影响因子：
16.2
通讯作者：
BERG, DK
中科院分区：
文献类型：
--
作者：
CONROY, WG;VERNALLIS, AB;BERG, DK
基于序列同源性，乙酰胆碱受体（AChR）α 5基因已被分类为AChR基因家族的成员。然而，表达研究还没有确定α 5基因产物的功能，甚至还没有证明与已知AChR亚基的相互作用。我们在这里报告的α 5基因产物是相同的49 kd蛋白质先前发现的免疫印迹纯化的乙酰胆碱受体从大脑和睫状神经节。在脑中，α 5基因产物存在于基于α 3和α 4的受体亚型中，而在神经节中，它存在于集中在突触后膜中的基于α 3的受体亚型中。亚基特异性单克隆抗体的免疫沉淀实验表明，一些天然AChRs可能至少有三种亚基，其中两种是α型。这些发现支持了关于AChRs在神经元中构建的新观点。
The acetylcholine receptor (AChR) alpha5 gene has been classified as a member of the AChR gene family based on sequence homology. Expression studies, however, have yet to identify a function for the alpha5 gene product or even to demonstrate an interaction with known AChR subunits. We report here that the alpha5 gene product is identical to the 49 kd protein previously found on immunoblots of AChRs purified from brain and ciliary ganglia. In brain the alpha5 gene product is present both in alpha3- and in alpha4-based receptor subtypes, while in the ganglion it is found in an alpha3-based receptor subtype concentrated in postsynaptic membrane. Immunoprecipitation experiments with subunit-specific monoclonal antibodies indicate that some native AChRs are likely to have at least three kinds of subunits, with two being of the alpha type. These findings support new views about the construction of AChRs in neurons.