Room temperature femtosecond X-ray diffraction of photosystem II microcrystals
Room temperature femtosecond X-ray diffraction of photosystem II microcrystals
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DOI：
10.1073/pnas.1204598109
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发表时间：
2012-06-19
影响因子：
11.1
通讯作者：
Yachandra, Vittal K.
中科院分区：
文献类型：
--
作者：
Kern, Jan;Alonso-Mori, Roberto;Yachandra, Vittal K.
地球上的大部分二氧化氧是由光系统II(PS II)利用来自太阳的光线氧化水产生的。这种光驱动的四光子反应是由位于PS II腔侧面的Mn4CaO5团簇催化的。人们在低温下进行了各种X射线研究，以了解水氧化机理中涉及的中间步骤。然而，需要在室温下收集数据，特别是为了研究O-O键形成过程中的瞬时步骤，需要开发新的方法。本文报道了用超短X射线衍射仪获得的PSⅡ微晶的室温X射线衍射数据。
Most of the dioxygen on earth is generated by the oxidation of water by photosystem II (PS II) using light from the sun. This lightdriven, four-photon reaction is catalyzed by the Mn4CaO5 cluster located at the lumenal side of PS II. Various X-ray studies have been carried out at cryogenic temperatures to understand the intermediate steps involved in the water oxidation mechanism. However, the necessity for collecting data at room temperature, especially for studying the transient steps during the O-O bond formation, requires the development of new methodologies. In this paper we report room temperature X-ray diffraction data of PS II microcrystals obtained using ultrashort (