DOG1-like genes in cereals: Investigation of their function by means of ectopic expression in Arabidopsis
DOG1-like genes in cereals: Investigation of their function by means of ectopic expression in Arabidopsis
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DOI：
10.1016/j.plantsci.2013.03.011
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发表时间：
2013-07-01
期刊：
影响因子：
5.2
通讯作者：
Nakamura, Shingo
中科院分区：
文献类型：
--
作者：
Ashikawa, Ikuo;Abe, Fumitaka;Nakamura, Shingo
拟南芥基因DOG 1（AtDOG 1）在种子休眠和糖信号中起作用。除了小麦族AtDOG 1-like基因（clade 1）与AtDOG 1功能上同源外，对AtDOG 1同源基因的结构和功能特征知之甚少。在这里，通过系统发育，结构和功能分析的谷物AtDOG 1样基因，我们的特点：这些基因作为一个基因家族，可以分为五个不同的分支（1-5）。其中，AtDOG 1-like基因在进化枝1-4中具有与AtDOG 1相似的结构：它们编码具有三个保守区域的蛋白质。相反，进化枝5基因是不同的;它们编码的蛋白质缺乏这些保守区域，但含有与DNA相互作用的结构域。在拟南芥中的分化枝2-4的谷物AtDOG 1-like基因的异位表达表明，像分化枝1的基因，它们执行相同的功能作为AtDOG 1。在转基因拟南芥中由进化枝1-4中的基因赋予的种子休眠深度和糖信号传导效率之间的相关性表明AtDOG 1的种子休眠和糖信号传导功能之间的潜在机制中存在密切联系。(C)2013爱思唯尔爱尔兰有限公司版权所有。
The Arabidopsis gene DOG1 (AtDOG1) functions in seed dormancy and in sugar signaling. Little is known about the structural and functional features of plant genes homologous to AtDOG1, except for one type (clade 1) of Triticeae AtDOG1-like genes, which was previously demonstrated to be functionally ortho-logous to AtDOG1. Here, through phylogenetic, structural, and functional analyses of cereal AtDOG1-like genes, we characterized their features: these genes exist as a gene family that can be classified into five distinct clades (1-5). Of these, AtDOG1-like genes in clades 1-4 have a similar architecture to AtDOG1: they encode proteins with three conserved regions. In contrast, the clade 5 genes are distinct; their encoded proteins lack these conserved regions, but harbor domains that interact with DNA. Ectopic expression of the cereal AtDOG1-like genes of clades 2-4 in Arabidopsis demonstrated that like the clade 1 genes, they performed the same function as AtDOG1. The correlation between the depth of seed dormancy and the efficiency of sugar signaling in transgenic Arabidopsis conferred by genes in clades 1-4 suggests a close link in the underlying mechanisms between the seed dormancy and sugar signaling functions of AtDOG1. (C) 2013 Elsevier Ireland Ltd. All rights reserved.