Analysis of HPV16, 18, 31, and 35 DNA in pre-invasive and invasive lesions of the uterine cervix
Analysis of HPV16, 18, 31, and 35 DNA in pre-invasive and invasive lesions of the uterine cervix
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DOI：
10.1136/jcp.50.7.600
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发表时间：
1997-07-01
影响因子：
3.4
通讯作者：
Becciolini, A
中科院分区：
文献类型：
--
作者：
Pirami, L;Giache, V;Becciolini, A
目的：分析55例宫颈上皮内瘤变和侵袭性宫颈肿瘤中不同类型HPV DNA的物理状态。方法对每例宫颈上皮内瘤变标本进行5种HPV型特异性酶的限制性内切酶分析，然后进行Southern杂交分析。结果：24例宫颈上皮内瘤变中6例(25%)整合了不同类型的HPV DNA。相比之下，在31例宫颈癌中有25例(81%)被检测到整合。肿瘤样本显示HPV16和其他类型的HPV的整合情况有所不同。23例HPV16相关癌中有6例(26%)仅含有上体DNA。相反，所有8个含有HPV18、31或35的肿瘤都显示出排他性的DNA整合。结论--结果表明，在晚期宫颈上皮内瘤变病变中，可以识别出病毒基因组整合的病变亚群，并且有更大的恶性进展风险。此外，在26%的肿瘤中，HPV16DNA没有以整合的形式存在，这表明转录调节因子E2的整合和随后的失活不是HPV16相关癌发展的必要步骤。在这方面，HPV16相关肿瘤的行为不同于HPV18、31和35相关肿瘤，在这些肿瘤中，病毒基因组总是以整合的形式存在。
Aims-To analyse the physical state of different human papillomavirus (HPV) DNAs in 55 intraepithelial and invasive HPV associated cervical neoplasms.Methods-Restriction analysis, using a panel of five HPV type specific enzymes, was carried out for each sample; this was followed by Southern blot analysis.Results-Six (25%) of 24 cervical intraepithelial neoplasms had integrated DNA of different HPV types. In contrast, integration was detected in 25 (81%) of 31 cervical carcinomas. Tumour samples revealed differences in the integration profile of HPV16 and the other HPV types. Six (26%) of 23 HPV16 associated cancers contained only episomal DNA. In contrast, all eight tumours containing HPV18, 31, or 35 revealed integrated DNA exclusively.Conclusions-The results suggest that in advanced cervical intraepithelial neoplasia lesions, a subset of lesions can be identified in which the viral genome is integrated and there is a greater risk of malignant progression. In addition, HPV16 DNA was not present in the integrated form in 26% of tumours, suggesting that integration and subsequent inactivation of the transcriptional regulator, E2, are not essential steps for the development of HPV16 associated carcinoma. In this respect, the behaviour of HPV16 associated tumours is different from HPV18, 31, and 35 associated tumours, where the viral genome is always present in the integrated form.