Analysis of the toxic mode of action of silver nanoparticles using stress-specific bioluminescent bacteria
Analysis of the toxic mode of action of silver nanoparticles using stress-specific bioluminescent bacteria
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DOI：
10.1002/smll.200700954
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发表时间：
2008-06-01
期刊：
影响因子：
13.3
通讯作者：
Gu, Man Bock
中科院分区：
文献类型：
--
作者：
Hwang, Ee Taek;Lee, Jin Hyung;Gu, Man Bock
随着纳米科学的发展，许多纳米粒子已被生产和应用于广泛的领域和应用，如电子、磁学和光电子、生物医学、制药、化妆品、能源、环境和一般材料的开发。尽管纳米颗粒用途广泛，但许多人对其对环境和人体健康的影响持怀疑态度，这一点在其他研究中也得到了审查和辩论。[1,2]用于细胞存活和增殖的MTT（比色法）和LDH测定通常用于评估纳米颗粒引起的毒性。[3-7]然而，纳米颗粒暴露后的细胞反应引起了研究人员的兴趣。在任何评估纳米颗粒及其毒性的研究中，都应该考虑几个参数，包括颗粒大小、团聚程度和比表面积。先前的几项研究表明，纳米颗粒的团聚影响毒性。[8,9]例如，Limbach等人在他们的研究中开发了一个描述颗粒聚集、扩散和沉积的模型该模型可用于评价各种纳米颗粒及其毒性的研究。银纳米粒子之所以引起人们的兴趣，是因为它们可以用作消毒剂。含银产品在全世界都很常见，而且数量还在不断增长。前人对银离子的杀菌作用进行了研究。[10-12]然而，据报道，纳米尺寸的颗粒具有不同的性质，并且由于其极小的尺寸，具有大的表面积和高的反应性
With the recent advances in the nanosciences, many nanoparticles have been produced and utilized in broad fields and applications, such as in electronics, magnetic and optoelectronics, biomedicine, pharmaceuticals, cosmetics, energy, the environment, and general materials development. In spite of their versatility, many people are suspicious of the effects nanoparticles have on both the environment and human health, and this has been reviewed and debated in other studies.[1, 2] The MTT (colorimetric) and LDH assays, for cell survival and proliferation, have been typically used to assess the toxicity caused by nanoparticles.[3–7] However, the cellular responses after exposure to nanoparticles are of interest to researchers. Several parameters exist that should be considered in any study that evaluates nanoparticles and their toxicity, including the particle size, degree of agglomeration, and specific surface area. The agglomeration of nanoparticles affects the toxicity, as demonstrated by several previous studies.[8, 9] For example, in their study, Limbach et al. developed a model that describes particle aggregation, diffusion, and sedimentation.[8] Such a model would be useful in studies evaluating various nanoparticles and their toxicity.Silver nanoparticles are of interest because of their use as disinfectants. Products containing silver are commonplace worldwide and continue to grow in number. The bactericidal action of the silver ion has been studied in previous studies.[10–12] However, it was reported that nanometer-sized particles have different properties and, because of their extremely small size, have a large surface area and high reactivity.[13]