Orally administered Bifidobacterium triggers immune responses following capture by CD11c+ cells in Peyer's patches and cecal patches
Orally administered Bifidobacterium triggers immune responses following capture by CD11c+ cells in Peyer's patches and cecal patches
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DOI：
10.1007/s10616-011-9349-6
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发表时间：
2011-05-01
期刊：
影响因子：
2.2
通讯作者：
Kaminogawa, Shuichi
中科院分区：
文献类型：
--
作者：
Hiramatsu, Yasuhiro;Hosono, Akira;Kaminogawa, Shuichi
本文研究了双歧杆菌JCM7041(BP)对小鼠口服益生菌的免疫调节机制。本研究旨在通过阐明口服细菌细胞在体内转移的详细机制以及细菌细胞与免疫组织细胞之间的相互作用来阐明口服益生菌诱导免疫调节的机制。我们观察了BP经口给药后在小鼠体内的定位，发现BP周围有CD11c(+)细胞包围在Peyer‘s斑块(PP)和盲肠斑块(CP)。提示BP可直接诱导CD11c(+)细胞免疫应答。此外，Thy1.2(-)细胞，包括CD11c(+)细胞产生的IL-10和IL-12p40显著增加。BP刺激可显著增加骨髓来源的树突状细胞(BMDC)产生IL-10和IL-12p40的能力。这些结果表明，口服BP被CD11c(+)树突状细胞(DC)捕获后立即诱导免疫反应。随后，我们观察到BP口服1周可诱导CP和PP细胞产生IgA和IgA相关细胞因子，提示BP可诱导CP和PP的DC介导的免疫应答。
We have investigated the immunomodulatory mechanisms of Bifidobacterium pseudocatenulatum JCM7041 (Bp) as model of probiotics following oral administration to mice. This study was conducted with the aim of clarifying the mechanism of immunomodulation induced by oral administration of probiotic bacteria through elucidation of the detailed mechanism of transfer of orally administered bacterial cells within the body and the interaction between bacterial cells and cells of the immune tissues. We observed the localization of Bp in mice following oral administration, showing that Bp was surrounded by CD11c(+) cells in Peyer's patches (PP) and cecal patches (CP). These results indicated that Bp might induce CD11c(+) cell-mediated immune responses directly. Furthermore, IL-10 and IL-12p40 production by Thy1.2(-) cells, including CD11c(+) cells, increased significantly. Production of IL-10 and IL-12p40 by bone marrow-derived dendritic cells (BMDC) was significantly increased by Bp stimulation. These results suggest that oral administration of Bp induces immune responses directly following capture by CD11c(+) dendritic cells (DCs). Subsequently, we observed oral administration of Bp for 1 week induced IgA and IgA-associated cytokine production by CP and PP cells, suggesting that Bp induced DC-mediated immune responses on CP as well as PP.