Hydroxyapatite-coated tendon chitosan tubes with adsorbed laminin peptides facilitate nerve regeneration in vivo
Hydroxyapatite-coated tendon chitosan tubes with adsorbed laminin peptides facilitate nerve regeneration in vivo
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DOI：
10.1016/j.brainres.2003.09.003
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发表时间：
2003-12-12
期刊：
影响因子：
2.9
通讯作者：
Tanaka, J
中科院分区：
文献类型：
--
作者：
Itoh, S;Yamaguchi, I;Tanaka, J
在具有三角形形状和羟基磷灰石涂层的肌腱壳聚糖管（t-chitosan/HAp管）的内表面上，层粘连蛋白-1和层粘连蛋白肽（YIGSR，IKVAV）已经被吸附，以形成神经生长管道。并对该材料的力学性能、生物相容性及神经再生效果进行了检测。第一步：使用具有圆形或三角形横截面的t-壳聚糖或t-壳聚糖/HAp管（每组N = 12）将桥接移植（15 mm）到Sprague-Dawley（SD）大鼠的坐骨神经中。在植入后2、4、6和8周（每组N = 3）采集标本进行组织学测定。第二步：具有三角形横截面的吸附有层粘连蛋白-1、CDPGYIGSR或CSRARKQAASIKVAVSAD的t-壳聚糖/HAp管以及没有预吸附的对照管用于植入（每组N = 18）。还进行了同种接枝（N = 6）。组织学评价与步骤-1中类似地进行。12周后，记录诱发的肌肉和感觉神经动作电位，并测量实验组、对照组和同系移植组（每组N = 6）在远端闭塞部位10 mm处有髓轴突面积的百分比。组织学结果以及机械性能的结果表明，具有HAp涂层的三角形管形状有利于神经再生。已发现层粘连蛋白肽（YIGSR，随后是IKVAV）增强再生轴突生长的作用与完整层粘连蛋白-1相当。尽管在YIGSR和层粘连蛋白-1处理的t-壳聚糖/HAp管中的组织学再生与同种移植物相匹配，但是功能恢复被延迟。(C)2003 Elsevier B. V.保留所有权利。
On the inner surface of tendon chitosan tubes having a triangular shape and a hydroxyapatite coating (t-chitosan/HAp tube), laminin-1 and laminin peptides (YIGSR, IKVAV) have been adsorbed in order to develop nerve growth conduits. The mechanical property, biocompatibility and efficacy of these tubes for nerve regeneration were examined. Step-1: bridge grafting (15 mm) into the sciatic nerve of Sprague-Dawley (SD) rats was carried out using either t-chitosan or t-chitosan/HAp tubes having either a circular or triangular cross section (N = 12 in each group). Specimens were taken after 2-, 4-, 6- and 8-week post-implantation (N = 3 in each group) for histology determinations. Step-2: t-chitosan/HAp tubes having a triangular cross section with adsorbed laminin-1, CDPGYIGSR or CSRARKQAASIKVAVSAD, as well as control tubes without pre-adsorption were used for implantation (N = 18 in each group). Isografting was also carried out (N = 6). Histological evaluation was carried out similarly as in Step-1. Furthermore, evoked muscle and sensory nerve action potentials were recorded, and the percentage of myelinated axon area measured at 10 mm distance of the distal anastomosed site in the experimental, control and isograft groups after 12 weeks (N = 6 in each group). The results of histological findings, as well as mechanical properties, suggest that a triangular tube shape with a HAp coating benefits nerve regeneration. The effect of laminin peptides (YIGSR, followed by IKVAV) to enhance the growth of regenerating axons has been found comparable with intact laminin-1. Although histological regeneration in both the YlGSR- and laminin-1-treated t-chitosan/HAp tubes matches the isografts, the functional recovery is however delayed. (C) 2003 Elsevier B.V. All rights reserved.