Complementary DNA Sequence of a Low Temperature-Induced Brassica napus Gene with Homology to the Arabidopsis thaliana kin1 Gene.
Complementary DNA Sequence of a Low Temperature-Induced Brassica napus Gene with Homology to the Arabidopsis thaliana kin1 Gene.
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DOI：
10.1104/pp.98.4.1532
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发表时间：
1992-04
期刊：
影响因子：
7.4
通讯作者：
W. Orr;B. Iu;T. White;L. Robert;Jas Singh
中科院分区：
文献类型：
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作者：
W. Orr;B. Iu;T. White;L. Robert;Jas Singh
低温触发能够发展抗寒性的植物物种获得抗冻性。最近，在苜蓿（9）、拟南芥（5，7，10）、大麦（2，3）和小麦（6，8）中观察到，在冷驯化期间，低温至少部分诱导了特定基因的转录。在小麦和拟南芥中，冷诱导的mRNA显示出指导热稳定多肽的翻译（6，8）;在水稻中，暴露于低温导致rab（响应于阿坝）基因产物的积累（4）。当植物暴露于低温时，编码这些基因产物的mRNA迅速出现，并且在大多数情况下，也可通过水分胁迫或直接施用阿坝诱导（5，7，10）。虽然这些冷调节基因的产物的确切功能尚未阐明，但与RAB或莱亚（晚期胚胎发生丰富）蛋白质在推导的氨基酸基序和相似的理化性质方面的相似性的证据表明，它们可能在保护细胞免受伴随细胞外冷冻的细胞脱水中发挥作用。从甘蓝型油菜（Brassica napus）cv 2°C中克隆了一个差异表达的Xgt 10文库，该文库是一个冷诱导的cDNA文库。
Low temperature triggers the acquisition of frost tolerance in plant species that are capable of developing cold hardiness. Recently, the transcription of specific genes was observed to be induced, at least in part, by low temperature in alfalfa (9), Arabidopsis (5, 7, 10), barley (2, 3), and wheat (6, 8) during cold acclimation. In wheat and Arabidopsis, the cold-induced mRNAs were shown to direct the translation of heat-stable polypeptides (6, 8); in rice, exposure to low temperatures resulted in the accumulation of rab (responsive to ABA) gene products (4). The mRNAs encoding these gene products appear rapidly when the plants are exposed to low temperature and, in the majority of cases, are also inducible by water stress or by the direct application of ABA (5, 7, 10). Although the exact function of the products of these cold-regulated genes has not been elucidated, evidence of similarity in deduced amino acid motifs and similar physicochemical properties with rab or lea (late embryogenic abundant) proteins suggests that they may play a role in protecting the cell from the cellular dehydration that accompanies extracellular freezing. Differential a XgtlO library, from the Brassica napus, cv 2°C, was the cDNA of a cold-induced We report cDNA deduced amino acid a low-temperature-regulated region nucleotide sequence (77%)