Diverse Sequence Types of Klebsiella pneumoniae Contribute to the Dissemination of blaNDM-1 in India, Sweden, and the United Kingdom
Diverse Sequence Types of Klebsiella pneumoniae Contribute to the Dissemination of blaNDM-1 in India, Sweden, and the United Kingdom
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DOI：
10.1128/aac.06142-11
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发表时间：
2012-05-01
影响因子：
4.9
通讯作者：
Walsh, Timothy R.
中科院分区：
文献类型：
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作者：
Giske, Christian G.;Froding, Inga;Walsh, Timothy R.
对来自印度(n=22)、英国(n=13)和瑞典(n=4)的产NDM-1碳青霉烯酶肺炎克雷伯菌临床分离株进行了多位点序列分型(MLST)、基于自动重复序列的聚合酶链式反应(REP-PCR)、血清分型、毒力基因筛选和质粒复制子分型。最常见的MLST序列类型(STS)是ST14(n=13，均为K2血清型)、ST11、ST149、ST231和ST147。MLST法与全自动REP-PCR法的相关性良好。Inca/C是最常见的质粒复制子类型(n=14)。ST14、ST11和其他成功的克隆可能对BLA(NDM-1)的传播很重要。
Clinical isolates of Klebsiella pneumoniae producing NDM-1 carbapenemase from India (n = 22), the United Kingdom (n = 13), and Sweden (n = 4) were subjected to multilocus sequence typing (MLST), automated repetitive sequence-based PCR (rep-PCR), serotyping, virulence gene screening, and plasmid replicon typing. The most frequently detected MLST sequence types (STs) were ST14 (n = 13; all serotype K2), ST11, ST149, ST231, and ST147. The correlation between MLST and automated rep-PCR was excellent. IncA/C was the most frequently detected plasmid replicon type (n = 14). ST14, ST11, and other successful clones may be important for the dissemination of bla(NDM-1).