Polaribacter sejongensis sp nov., isolated from Antarctic soil, and emended descriptions of the genus Polaribacter, Polaribacter butkevichii and Polaribacter irgensii
Polaribacter sejongensis sp nov., isolated from Antarctic soil, and emended descriptions of the genus Polaribacter, Polaribacter butkevichii and Polaribacter irgensii
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DOI：
10.1099/ijs.0.047100-0
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发表时间：
2013-11-01
影响因子：
2.8
通讯作者：
Bae, Kyung Sook
中科院分区：
文献类型：
--
作者：
Kim, Byung-Chun;Oh, Hyun Woo;Bae, Kyung Sook
从南极土壤中分离到一株革兰氏染色阴性、过氧化氢酶和氧化酶阳性的非游动细菌，命名为KOPRI 21160(T)。经16SRRNA基因序列分析，菌株KOPRI 21160(T)属于Polaribacter属。KOPRI 21160(T)菌株与Polaribacter属模式菌株的序列相似性为94.2%~98.3%。KOPRI 21160(T)菌株的系统发育近邻是Polaribacter bukevichii KCTC 12100(T)(98.3%)和Polaribacter irgensii KCTC 23136(T)(97.5%)。KOPRI 21160(T)菌株与巴氏假单胞菌KCTC 12100(T)之间的DNADNA同源性为50.6%，KOPRI 21160(T)菌株与P.irgensii KCTC 23136(T)之间的DNADNA同源性为45.2%。菌株KOPRI 21160(T)在4-37℃、PH7.0-8.5时生长。它可以降解DNA、淀粉和青少年20、40、60和80岁。MK-6为唯一的呼吸对苯二酚，细胞内主要脂肪酸为iso-C-15：0、iso-C-15：03-OH和C-15：1 omega 6c。主要的极性脂类是磷脂酰乙醇胺、两种未知的氨基脂和一种未知的脂类。DNA G+C含量为30.0mol%。根据我们多相研究的数据，该菌被认为代表了Polaribacter属的一个新种，为此我们提出了Polaribacter sejongensis sp.11月模式菌株为KOPRI 21160(T)(=KCTC 23670(T)=JCM 18092(T))。对Polaribacter、Polaribacter bukevichii Nedashkovskaya等进行了订正。2005和Polaribacter irgensii Gosink等1998年。
A Gram-staining-negative, catalase- and oxidase-positive, non-motile bacterium, designated strain KOPRI 21160(T), was isolated from Antarctic soil. Based on 16S rRNA gene sequence analysis, strain KOPRI 21160(T) was found to belong to the genus Polaribacter. Sequence similarity between strain KOPRI 21160(T) and the type strains of species of the genus Polaribacter was 94.2-98.3%. The nearest phylogenetic neighbours of strain KOPRI 21160(T) were Polaribacter butkevichii KCTC 12100(T) (98.3% similarity) and Polaribacter irgensii KCTC 23136(T) (97.5%). DNA DNA relatedness was 50.6%, between strain KOPRI 21160(T) and P. butkevichii KCTC 12100(T), and 45.2% between strain KOPRI 21160(T) and P. irgensii KCTC 23136(T). Strain KOPRI 21160(T) grew at 4-37 degrees C and at pH 7.0-8.5. It could hydrolyse DNA, starch and Tweens 20, 40, 60 and 80. Menaquinone-6 (MK-6) was the only respiratory quinone, and iso-C-15:0, iso-C-15:0 3-OH and C-15:1 omega 6c were the major cellular fatty acids. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and one unidentified lipid. The DNA G+C content was 30.0 mol%. Based on data from our polyphasic study, the organism is considered to represent a novel species of the genus Polaribacter, for which we propose the name Polaribacter sejongensis sp. nov. The type strain is KOPRI 21160(T) (=KCTC 23670(T)=JCM 18092(T)). Emended descriptions of the genus Polaribacter, Polaribacter butkevichii Nedashkovskaya et al. 2005 and Polaribacter irgensii Gosink et aL 1998 are also proposed.