GROWTH AND ACTIVITIES OF SULFATE-REDUCING BACTERIA IN GUT CONTENTS OF HEALTHY-SUBJECTS AND PATIENTS WITH ULCERATIVE-COLITIS
GROWTH AND ACTIVITIES OF SULFATE-REDUCING BACTERIA IN GUT CONTENTS OF HEALTHY-SUBJECTS AND PATIENTS WITH ULCERATIVE-COLITIS
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DOI：
10.1111/j.1574-6968.1991.tb04799.x
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发表时间：
1991-12-01
影响因子：
4.2
通讯作者：
MACFARLANE, GT
中科院分区：
文献类型：
--
作者：
GIBSON, GR;CUMMINGS, JH;MACFARLANE, GT
在人类大肠发酵过程中，末端氧化过程可能涉及异化硫酸盐还原菌（SRB）的活动。大约 50% 的健康个体的粪便中含有大量 SRB。在混合培养中，SRB 的体外生长受到硫酸盐有效性的调节，与淀粉、果胶和阿拉伯半乳聚糖相比，硫酸化多糖（例如粘蛋白、硫酸软骨素和角叉菜胶）会导致生长速率和硫化物产量增加。与健康志愿者相比，从溃疡性结肠炎患者中分离出的 SRB 中 H2S 的产生率更高。粪便样本中的大部分（高达 92%）SRB 属于脱硫弧菌属。体外研究表明，与来自健康受试者的分离株相比，来自结肠炎个体的脱硫弧菌能够更好地适应高稀释率，这可能与该疾病有关。这些发现表明，从人类大肠中分离出的 SRB 的代谢能力并不统一，可能会对肠道中可用的底物类型以及食糜的通过速率做出反应。
During fermentation in the human large intestine, terminal oxidative processes may involve the activities of dissimilatory sulphate-reducing bacteria (SRB). Approximately 50% of healthy individuals harbour significant populations of SRB in faeces. In mixed culture, growth of SRB in vitro was modulated by sulphate availability, with sulphated polysaccharides such as mucin, chondroitin sulphate and carrageenan causing increased growth rates and sulphide production when compared with starch, pectin and arabinogalactan. Rates of H2S production were higher among SRB isolated from patients with ulcerative colitis in contrast to those present in healthy volunteers. The majority (up to 92%) of SRB in faecal samples belonged to the genus Desulfovibrio. In vitro studies demonstrated that compared to isolates from healthy subjects, Desulfovibrio desulfuricans from colitic individuals were better able to adapt to high dilution rates, which may be associated with the disease. These findings indicate that the metabolic capabilities of SRB isolated from the human large intestine are not uniform and may respond to the type of substrate available in the gut as well as the rate of passage of digesta.