Interfacial Serum Protein Effect on Biological Apatite Growth
Interfacial Serum Protein Effect on Biological Apatite Growth
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DOI：
10.1021/jp208104z
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发表时间：
2011-10
影响因子：
3.7
通讯作者：
M. Tagaya;T. Ikoma;M. Takeguchi;N. Hanagata;J. Tanaka
中科院分区：
文献类型：
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作者：
M. Tagaya;T. Ikoma;M. Takeguchi;N. Hanagata;J. Tanaka
用石英晶体微天平(QCM-D)技术研究了模拟体液在不同表面(金(Au)、钛(Ti)和羟基磷灰石(HAP))上生长的生物磷灰石(BaP)在初始成核阶段有无预吸附胎牛血清蛋白(FBS)的情况。分别用Voigt粘弹性模型和抗体结合分析研究了FBS附着层的蛋白质组成和粘弹性。裸露的羟基磷灰石有效地促进了BaP的生长，在40h内质量增加到(2 5.9±3.2)μg cm-2，而在Au和Ti表面的质量仅增加了不到1μg cm-2，显示出良好的生物相容性。与HAP相比，FBS在裸金和钛上的吸附迅速达到平台期，并且在HAP上的吸附量大约是在Au和Ti上的2倍。羟基磷灰石上的纤维增强体具有较高的饱和ΔD/Δf值。
Biological apatite (BAp) growth from a simulated body fluid on various substrate surfaces (gold (Au), titanium (Ti), and hydroxyapatite (HAp)) with and without preadsorption of fetal bovine serum protein (FBS) at the initial nucleation stage was investigated using a quartz crystal microbalance with the dissipation (QCM-D) technique. The protein composition in the FBS adlayer and viscoelastic property was elucidated by the Voigt-based viscoelastic model and antibody-binding analysis, respectively. The bare HAp effectively induced the BAp growth to increase the mass to 25.9 ± 3.2 μg cm–2 in 40 h; however, a slight increased mass of less than 1 μg cm–2 was detected on the Au and Ti, indicating the superior biocompatibility of the HAp surface. The FBS adsorption on the bare Au and Ti rapidly reached a plateau as compared to that on the HAp, and the adsorbed amount of the FBS on the HAp was approximately 2 times greater than those on the Au and Ti. The FBS adlayer on HAp showed the higher saturated ΔD/Δf value...